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PMID: 3088203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequences and repertoire of human T cell receptor alpha chain variable region genes in mature T lymphocytes.

The Journal of experimental medicine ·Vol. 164 ·No. 1 ·1986-07-01 ·Pages 90-103

Yoshikai Y, Kimura N, Toyonaga B, Mak TW

Abstract

24 human T cell receptor alpha chain messages have been examined by cDNA sequence analysis and Southern blot. The data indicate that there are approximately 40 alpha chain T cell receptor variable gene segments, which can be divided into 12 families. Comparison of the J gene segments from the cDNAs to previously determined germline J alpha sequences places the number of J alpha gene segments over 21, and indicates their number to be approximately 55. Identical nucleotide sequences in independent isolates of V alpha and J alpha gene segments indicate that hypermutation may not be a common mechanism for the expansion of diversity in these genes, and suggest that the major source of diversity within the alpha chain repertoire is a result of recombinational joinings between germline V alpha and J alpha sequences, combined with imprecise junctional joining. Analysis of the V regions of these alpha chain messages reveals the presence of three domains of hypervariability roughly analogous to the CDR1, CDR2, and CDR3 regions of immunoglobulin.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Collodion DNA/isolation & purification Electrophoresis, Agar Gel Genes Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin alpha-Chains/genetics Nucleic Acid Hybridization Receptors, Antigen, T-Cell/genetics
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region Immunoglobulin alpha-Chains Receptors, Antigen, T-Cell Collodion DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yoshikai Y
Kimura N
Toyonaga B
Mak T W
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25 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.164.1.90
Published
1986-07-01
Pages
90-103
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188198
Subset
IM
Databases
GENBANK
M13722, M13723, M13724, M13725, M13726, M13727, M13728, M13729, M13730, M13731, M13732, M13733, M13734, M13735, M13736, M13737, M13738, M13739, M13740, M13741, M13742, M13743, M13744, M13745
External Links
PubMed source DOI: 10.1084/jem.164.1.90 PMC Full Text View journal details
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