The cloning and characterization of a RAS gene from Schizosaccharomyces pombe.

Journal of molecular evolution ·Vol. 23 ·No. 1 ·1986-00-00 ·Pages 41-51

Nadin-Davis SA, Yang RC, Narang SA, Nasim A

Abstract

We have cloned and determined the complete nucleotide sequence of a RAS gene from the yeast Schizosaccharomyces pombe (SP-RAS). The putative RAS protein of 214 amino acids is encoded by two noncontiguous reading frames separated by an intron of 86 bp. The SP-RAS gene product shares extensive homology with the proteins of the Saccharomyces cerevisiae (SC), Dictyostelium, Drosophila, and human RAS genes in its N-terminal region but not in its C-terminal region. The extended C-terminal regions found in the SC-RAS genes have no counterpart in the SP-RAS gene. Thus the RAS genes of these two yeasts are structurally quite distinct. The SP-RAS sequence was expressed in vivo.

MeSH Terms

Base Sequence Cloning, Molecular DNA, Recombinant/analysis Dictyostelium/genetics Drosophila/genetics Fungal Proteins/genetics Genes, Fungal Humans Neoplasm Proteins/genetics Oncogene Protein p21(ras) Oncogenes Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins p21(ras) Saccharomyces cerevisiae/genetics Saccharomycetales/genetics Schizosaccharomyces/genetics Sequence Homology, Nucleic Acid ras Proteins

Chemicals

DNA, Recombinant Fungal Proteins Neoplasm Proteins Proto-Oncogene Proteins ras protein, Dictyostelium HRAS protein, human Oncogene Protein p21(ras) Proto-Oncogene Proteins p21(ras) ras Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Nadin-Davis S A

Yang R C

Narang S A

Nasim A

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Article Info

Journal

Journal of molecular evolution

Abbr.

J Mol Evol

ISSN

0022-2844

DOI

10.1007/BF02100997

Published

1986-00-00

Pages

41-51

Language

English

Region

Germany

NLM ID

0360051

Subset

Databases

GENBANK

X03771

External Links

PubMed source DOI: 10.1007/BF02100997 View journal details

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The cloning and characterization of a RAS gene from Schizosaccharomyces pombe.

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