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PMID: 3079768 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for two physiologically distinct gap junctions expressed by the chick lens epithelial cell.

The Journal of cell biology ·Vol. 102 ·No. 1 ·1986-01-00 ·Pages 194-9

Miller TM, Goodenough DA

Abstract

Lens epithelial cells communicate with two different cell types. They communicate with other epithelial cells via gap junctions on their lateral membranes, and with fiber cells via junctions on their apices. We tested independently these two routes of cell-cell communication to determine if treatment with a 90% CO2-equilibrated medium caused a decrease in junctional permeability; the transfer of fluorescent dye was used as the assay. We found that the high-CO2 treatment blocked intraepithelial dye transfer but not fiber-to-epithelium dye transfer. The lens epithelial cell thus forms at least two physiologically distinct classes of gap junctions.

MeSH Terms
Animals Carbon Dioxide/pharmacology Cell Communication/drug effects Chickens Epithelial Cells Epithelium/physiology Fluorescent Dyes/metabolism Freeze Fracturing Intercellular Junctions/drug effects,physiology Lens, Crystalline/cytology,embryology
Chemicals
Fluorescent Dyes Carbon Dioxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miller T M
Goodenough D A
References (37)
37 references, click to expand
  1. Electrophysiological evidence for low-resistance intercellular junctions in the early chick embryo.
    J Cell Biol. 1968 Jun;37(3):650-9 PMID: 11905198
  2. Electrotonic coupling in internally perfused crayfish segmented axons.
    J Physiol. 1981 Aug;317:509-18 PMID: 6796679
  3. Gap junctions between electrotonically coupled cells in tissue culture and in brown fat.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):2924-7 PMID: 5289236
  4. Metabolic coupling, ionic coupling and cell contacts.
    Nature. 1972 Feb 4;235(5336):262-5 PMID: 4551177
  5. Three types of gap junctions interconnecting intestinal epithelial cells visualized by freeze-etching.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1318-21 PMID: 4504340
  6. Homocellular and heterocellular gap junctions in Limulus: a thin-section and freeze-fracture study.
    J Ultrastruct Res. 1973 May;43(3):298-312 PMID: 4575714
  7. Electrical coupling across developmental boundaries in insect epidermis.
    Nature. 1973 Sep 7;245(5419):47-8 PMID: 4583131
  8. The membrane junctions in communicating and noncommunicating cells, their hybrids, and segregants.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):880-4 PMID: 4522798
  9. Intercellular communication in a positional field: movement of small ions between insect epidermal cells.
    Dev Biol. 1974 Oct;40(2):311-22 PMID: 4430411
  10. Intercellular junctions in the early human embryonic retina.
    J Ultrastruct Res. 1975 Apr;51(1):69-78 PMID: 1127794
  11. Atypical gap junctions in the ciliary epithelium of the albino rabbit eye.
    J Cell Biol. 1975 Sep;66(3):671-6 PMID: 1158976
  12. Fine structure of the rectum in cockroaches (Dictyoptera): general organization and intercellular junctions.
    Tissue Cell. 1976;8(2):345-68 PMID: 941139
  13. Tight and gap junctions in a vertebrate inner ear.
    Am J Anat. 1976 Nov;147(3):281-301 PMID: 983970
  14. Gap junctions between the oocyte and companion follicle cells in the mammalian ovary.
    J Cell Biol. 1976 Nov;71(2):680-6 PMID: 825522
  15. A portrait of plasma membrane specializations in eye lens epithelium and fibers.
    Biochim Biophys Acta. 1976 Dec 14;457(3-4):353-84 PMID: 793636
  16. Selective contact-dependent cell communication.
    Nature. 1976 Dec 23-30;264(5588):760-2 PMID: 1012316
  17. A study of communication specificity between cells in culture.
    J Cell Biol. 1977 Dec;75(3):769-87 PMID: 562887
  18. Structural diversity of gap junctions. A review.
    Tissue Cell. 1977;9(3):373-94 PMID: 73230
  19. Calcium effects on gap junction structure and cell coupling.
    Nature. 1978 Feb 16;271(5646):669-71 PMID: 625335
  20. Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer.
    Cell. 1978 Jul;14(3):741-59 PMID: 688392
  21. Gap junctions of chick lens fiber cells.
    Exp Eye Res. 1978 Oct;27(4):495-8 PMID: 729657
  22. Selectivity in metabolic cooperation between cultured mammalian cells.
    Exp Cell Res. 1979 May;120(2):307-20 PMID: 436960
  23. Permeability of the cell-to-cell membrane channels in mammalian cell juncton.
    Science. 1979 Jul 27;205(4404):404-7 PMID: 377490
  24. Asymmetrically permeable membrane channels in cell junction.
    Science. 1980 Feb 15;207(4432):771-3 PMID: 7352287
  25. Comparative analysis of the major polypeptides from liver gap junctions and lens fiber junctions.
    J Cell Biol. 1982 Jan;92(1):53-9 PMID: 6173389
  26. Permeability of gap junctions at the segmental border in insect epidermis.
    Cell. 1982 Feb;28(2):243-52 PMID: 7060129
  27. Gap junctional conductance: comparison of sensitivities to H and Ca ions.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):441-5 PMID: 6281771
  28. Dye transfer between cells of the embryonic chick lens becomes less sensitive to CO2 treatment with development.
    J Cell Biol. 1982 Mar;92(3):694-705 PMID: 6806303
  29. Connections between cells of the developing squid as revealed by electrophysiological methods.
    Proc Natl Acad Sci U S A. 1966 Feb;55(2):328-36 PMID: 5220950
  30. Rotary replication of lens gap junction.
    J Ultrastruct Res. 1982 Nov;81(2):249-56 PMID: 7143550
  31. Differences between liver gap junction protein and lens MIP 26 from rat: implications for tissue specificity of gap junctions.
    Cell. 1983 Mar;32(3):967-78 PMID: 6299583
  32. The electrical coupling of epithelium and fibers in the frog lens.
    Exp Eye Res. 1983 Mar;36(3):317-26 PMID: 6601018
  33. Comparative analysis of the gap junction protein from rat heart and liver: is there a tissue specificity of gap junctions?
    Cell. 1983 Dec;35(2 Pt 1):539-49 PMID: 6317197
  34. Gap junction structures after experimental alteration of junctional channel conductance.
    J Cell Biol. 1985 Nov;101(5 Pt 1):1741-8 PMID: 2414303
  35. Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.
    J Cell Biol. 1980 Aug;86(2):576-89 PMID: 6772650
  36. Fine structure of the afferent synapse and gap junctions on the sensory hair cell in the saccular macula of goldfish: a freeze-fracture study.
    J Neurocytol. 1980 Dec;9(6):845-60 PMID: 6110710
  37. Permeability and structural studies of heart cell gap junctions under normal and altered ionic conditions.
    J Membr Biol. 1982;68(3):227-38 PMID: 6813505
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.102.1.194
Published
1986-01-00
Pages
194-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114033
Subset
IM
Grants
NEI NIH HHS · EY02430 · United States
NIGMS NIH HHS · GM18974 · United States
External Links
PubMed source DOI: 10.1083/jcb.102.1.194 PMC Full Text View journal details
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