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PMID: 3072098 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The allosuppressor gene SAL4 encodes a protein important for maintaining translational fidelity in Saccharomyces cerevisiae.

Current genetics ·Vol. 14 ·No. 6 ·1988-12-00 ·Pages 537-43

Crouzet M, Izgu F, Grant CM, Tuite MF

Abstract

Allosuppressor (sal) mutations enhance the efficiency of the yeast ochre suppressor SUQ5 and define five unlinked loci, SAL1-SAL5. A number of sal4 mutants were isolated and found to have pleiotropic, allele;specific phenotypes, including hypersensitivity in vivo to paromomycin and other antibiotics that stimulate translational errors in yeast. To examine further the nature of the SAL4 gene product, the wild type SAL4 gene was isolated by complementation of a conditional lethal allele sal4-2, and demonstrated to be a single copy gene encoding a single 1.6 kb transcript. Restriction mapping and DNA hybridisation analysis were used to demonstrate that the SAL4 gene is identical to the previously identified omnipotent suppressor gene SUP45 (SUP1). Our results implicate the SAL4 gene product as playing a major role in maintaining translational accuracy in yeast.

MeSH Terms
Anti-Bacterial Agents/pharmacology Blotting, Northern Blotting, Southern Cycloheximide/pharmacology DNA Probes DNA, Fungal/analysis Fungal Proteins/genetics Genes, Fungal Gentamicins/pharmacology Hygromycin B/pharmacology Nucleic Acid Hybridization Paromomycin/pharmacology Phenotype Protein Biosynthesis RNA, Fungal/analysis Restriction Mapping Saccharomyces cerevisiae/drug effects,genetics Suppression, Genetic
Chemicals
Anti-Bacterial Agents DNA Probes DNA, Fungal Fungal Proteins Gentamicins RNA, Fungal Hygromycin B Paromomycin Cycloheximide antibiotic G 418
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Crouzet M
Biological Laboratory, University of Canterbury, Kent, UK.
Izgu F
Grant C M
Tuite M F
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30 references, click to expand
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00434078
Published
1988-12-00
Pages
537-43
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00434078 View journal details
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