Genetic screen for cloned release factor genes.

Journal of bacteriology ·Vol. 158 ·No. 1 ·1984-04-00 ·Pages 362-4

Weiss RB, Murphy JP, Gallant JA

Abstract

Nonsense suppression reflects competition between a nonsense suppressor tRNA and a translational release factor. This provides a simple way to screen for release factor genes cloned into a multicopy plasmid. We have confirmed that the expected competition occurs with the gene for release factor 2, cloned by C.T. Caskey et al. (C.T. Caskey, W.C. Forrester, W. Tate, and C.D. Ward, J. Bacteriol. 158:365-368, 1984), and used it to clone the gene for release factor 1.

MeSH Terms

Cloning, Molecular Codon Escherichia coli/genetics Genes, Bacterial Peptide Termination Factors/genetics Plasmids Protein Biosynthesis RNA, Bacterial/genetics RNA, Transfer/genetics Suppression, Genetic

Chemicals

Codon Peptide Termination Factors RNA, Bacterial peptide chain termination release factor 2 RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Weiss R B

Murphy J P

Gallant J A

References (10)

10 references, click to expand

Release factors differing in specificity for terminator codons.
Proc Natl Acad Sci U S A. 1968 Oct;61(2):768-74 PMID: 4879404
Characterization of three proteins involved in polypeptide chain termination.
Cold Spring Harb Symp Quant Biol. 1969;34:469-77 PMID: 4909514
Peptide chain termination, codon, protein factor, and ribosomal requirements.
Cold Spring Harb Symp Quant Biol. 1969;34:479-88 PMID: 4909515
Release factor translation of RNA phage terminator codons.
Nature. 1970 Jul 4;227(5253):38-40 PMID: 5422622
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
Simple method for identification of plasmid-coded proteins.
J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
Nonsense suppression in aminoacyl-t-RNA limited cells.
Mol Gen Genet. 1982;186(2):221-7 PMID: 6180282
Cloning of the Escherichia coli release factor 2 gene.
J Bacteriol. 1984 Apr;158(1):365-8 PMID: 6370964
SUBSTRATES FOR CYTOCHEMICAL DEMONSTRATION OF ENZYME ACTIVITY. I. SOME SUBSTITUTED 3-INDOLYL-BETA-D-GLYCOPYRANOSIDES.
J Med Chem. 1964 Jul;7:574-5 PMID: 14221156

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.158.1.362-364.1984

Published

1984-04-00

Pages

362-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215426

Subset

External Links

PubMed source DOI: 10.1128/jb.158.1.362-364.1984 PMC Full Text View journal details

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