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PMID: 3070349 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Random cloning of bent DNA segments from Saccharomyces cerevisiae and primary characterization of their structures.

Molecular & general genetics : MGG ·Vol. 214 ·No. 2 ·1988-10-00 ·Pages 249-56

Mizuno T, Itoh K

Abstract

Until recently it was assumed that any short segment of DNA could be approximated as a straight rod. Many instances, however, have been reported in which the helical axis is curved. We have devised a simple method for selective identification of DNA segments containing a sequence-directed bend (curvature), by means of a two-dimensional polyacrylamide gel electrophoresis. In order to gain general insights into the structural features and the functional significance of sequence-directed bends, a bank of plasmids carrying bent DNA inserts from the Saccharomyces cerevisiae total genomic DNA was constructed. Primary characterizations of a set of bent DNA segments randomly cloned from S. cerevisiae are presented. One of the cloned DNA segments appears to be derived from a yeast plasmid, the 2 microns circle DNA.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Circular/genetics DNA, Fungal/genetics Escherichia coli/genetics Molecular Sequence Data Nucleic Acid Conformation Plasmids Saccharomyces cerevisiae/genetics
Chemicals
DNA, Circular DNA, Fungal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mizuno T
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Itoh K
References (24)
24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337718
Published
1988-10-00
Pages
249-56
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337718
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