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PMID: 3066683 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation of yeast by agitation with glass beads.

Genetics ·Vol. 120 ·No. 3 ·1988-11-00 ·Pages 667-70

Costanzo MC, Fox TD

Abstract

We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal requirements for transformation are simply physical damage and the presence of DNA in an osmotically supportive environment suggests that this process may occur in nature.

MeSH Terms
Cell Cycle Cell Membrane Permeability DNA/genetics Genetic Techniques Glass Plasmids Saccharomyces cerevisiae/genetics,growth & development Transformation, Genetic
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Costanzo M C
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
Fox T D
References (13)
13 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/120.3.667
Published
1988-11-00
Pages
667-70
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203545
Subset
IM
Grants
NIGMS NIH HHS · GM29362 · United States
NICHD NIH HHS · HD00515 · United States
External Links
PubMed source DOI: 10.1093/genetics/120.3.667 PMC Full Text View journal details
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