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PMID: 3049078 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The export of the DNA replication inhibitor Microcin B17 provides immunity for the host cell.

The EMBO journal ·Vol. 7 ·No. 6 ·1988-06-00 ·Pages 1853-62

Garrido MC, Herrero M, Kolter R, Moreno F

Abstract

Microcin B17 (MccB17) is a peptide antibiotic which inhibits DNA replication in Enterobacteriaceae. Microcin-producing strains are immune to the action of the microcin. Physical and genetic studies showed that immunity is mediated by three genes: mcbE, mcbF and mcbG. We sequenced these genes and identified polypeptide products for mcbF and mcbG. By studying the contribution of each gene to the expression of immunity we found that immunity is determined by two different mechanisms. One of these, encoded by mcbE and mcbF, is also involved in the production of extracellular MccB17. To reconcile these observations we propose that McbE and McbF serve as a 'pump' for the export of active MccB17 from the cytoplasm. This model is supported by the predicted properties of the McbE and McbF proteins, which are thought to be, respectively, an integral membrane protein and an ATP-binding protein with homology to other transport proteins.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,physiology Bacteriocins/genetics,metabolism,physiology Base Sequence Biological Transport DNA Replication/drug effects DNA, Bacterial/drug effects Escherichia coli/genetics,physiology Escherichia coli Proteins Genes, Bacterial Membrane Proteins Membrane Transport Proteins Models, Biological Molecular Sequence Data SOS Response, Genetics
Chemicals
Bacterial Proteins Bacteriocins DNA, Bacterial Escherichia coli Proteins McbE protein, E coli McbF protein, E coli McbG protein, E coli Membrane Proteins Membrane Transport Proteins microcin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garrido M C
Uuidad de Genetica Molecular, Hospital Ramón y Cajal, Madrid, Spain.
Herrero M
Kolter R
Moreno F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-06-00
Pages
1853-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457178
Subset
IM
Databases
GENBANK
X07875
External Links
PubMed source PMC Full Text View journal details
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