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PMID: 3047680 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The UAS of the yeast PGK gene is composed of multiple functional elements.

Nucleic acids research ·Vol. 16 ·No. 17 ·1988-09-12 ·Pages 8245-60

Chambers A, Stanway C, Kingsman AJ, Kingsman SM

Abstract

The UAS (upstream activator sequence) of the yeast PGK gene contains a transcriptional activator domain located between bases -479 and -402 upstream from the initiating ATG. This region of the UAS contains three direct repeats of the sequence 5'CTTCC3'. The roles in transcriptional activation of these repeats and other sequences within the activator domain were investigated. When short regions containing the repeats were removed PGK expression was considerably reduced, the magnitude of the effect depended upon which CTTCC block was absent. Sequences between -473 and -458 which did not contain a CTTCC block were also shown to be necessary for high levels of PGK expression. A DNA fragment containing activator sequences up to -473 was shown to interact specifically in vitro with a yeast nuclear protein extract. DNase I footprinting identified a protected region between -473 and -458 and single base changes in DNase I sensitivity at the CTTCC repeats.

MeSH Terms
Chromosome Deletion Escherichia coli/genetics Genes Genes, Fungal Phosphoglycerate Kinase/genetics Promoter Regions, Genetic Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic
Chemicals
Phosphoglycerate Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chambers A
Department of Biochemistry, University of Oxford, UK.
Stanway C
Kingsman A J
Kingsman S M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.17.8245
Published
1988-09-12
Pages
8245-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338556
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.17.8245 PMC Full Text View journal details
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