Home LiteratureArticle Details
PMID: 3041373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and sequence of gene dicB: translation of the division inhibitor from an in-phase internal start.

Nucleic acids research ·Vol. 16 ·No. 14A ·1988-07-25 ·Pages 6327-38

Cam K, Béjar S, Gil D, Bouché JP

Abstract

The dicA1 mutation, located in the replication termination region of Escherichia coli at 34.9 min, confers a temperature-sensitive, division defective phenotype to its hosts. Previous analysis had suggested that dicA codes for a repressor of a nearby division inhibition gene dicB. We show now that gene dicB is part of a complex operon. Five open reading frames (ORFs 1 to 5) preceeded by a promoter sensitive to dicA repression are found within a 1500 bp segment, and are organized into two clusters separated by a long untranslated region. Evidence for expression of these ORFs was obtained from in vitro or in vivo translation of plasmid-coded genes. IPTG-dependent cell filamentation was obtained when either the entire or the C-terminal part of the fourth ORF was placed under control of the lac promoter. In both cases, a 7 KD protein corresponding to translation from an in-frame ATG of ORF4 (dicB) was made. We propose that this C-terminal protein is the division inhibitor synthesized in dicA1 mutants.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Division Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Operon Plasmids Transformation, Genetic
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cam K
Centre de Recherches de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
Béjar S
Gil D
Bouché J P
References (29)
29 references, click to expand
  1. A technique for integrating any DNA fragment into the chromosome of Escherichia coli.
    Gene. 1984 Jul-Aug;29(1-2):231-41 PMID: 6092225
  2. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  3. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.
    Gene. 1984 Nov;31(1-3):165-71 PMID: 6098521
  4. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  5. Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene.
    EMBO J. 1985 Apr;4(4):1059-66 PMID: 2990907
  6. A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli.
    Mol Gen Genet. 1985;201(2):146-50 PMID: 3911021
  7. Control of cell division in Escherichia coli. DNA sequence of dicA and of a second gene complementing mutation dicA1, dicC.
    Nucleic Acids Res. 1986 Sep 11;14(17):6821-33 PMID: 3532030
  8. Nucleolytic processing of ribonucleic acid transcripts in procaryotes.
    Microbiol Rev. 1986 Dec;50(4):428-51 PMID: 2432388
  9. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1754-8 PMID: 3550796
  10. Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1759-63 PMID: 3550797
  11. Cell division inhibition gene dicB is regulated by a locus similar to lambdoid bacteriophage immunity loci.
    Mol Gen Genet. 1988 Apr;212(1):11-9 PMID: 2836697
  12. Genetic recombination in Escherichia coli: the role of exonuclease I.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):824-7 PMID: 4927675
  13. In vitro synthesis of protein in microbial systems.
    Annu Rev Genet. 1973;7:267-87 PMID: 4593305
  14. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  15. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  16. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  17. Shotgun DNA sequencing using cloned DNase I-generated fragments.
    Nucleic Acids Res. 1981 Jul 10;9(13):3015-27 PMID: 6269069
  18. Physical map of a 470 x 10(3) base-pair region flanking the terminus of DNA replication in the Escherichia coli K12 genome.
    J Mol Biol. 1982 Jan 5;154(1):1-20 PMID: 6281436
  19. Relationships between the physical and genetic maps of a 470 x 10(3) base-pair region around the terminus of Escherichia coli K12 DNA replication.
    J Mol Biol. 1982 Jan 5;154(1):21-32 PMID: 6281437
  20. Temperature-sensitive copy number mutants of CoIE1 are located in an untranslated region of the plasmid genome.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3570-4 PMID: 6179081
  21. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
    Gene. 1982 Jun;18(3):199-209 PMID: 6751939
  22. Escherichia coli ribonuclease III cleavage sites.
    Cell. 1982 Oct;30(3):669-72 PMID: 6754088
  23. Translational coupling at an intercistronic boundary of the Escherichia coli galactose operon.
    Cell. 1982 Oct;30(3):865-71 PMID: 6754091
  24. Recognition of protein coding regions in DNA sequences.
    Nucleic Acids Res. 1982 Sep 11;10(17):5303-18 PMID: 7145702
  25. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  26. Use of statistical criteria for screening potential homologies in nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):203-13 PMID: 6694901
  27. Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):789-800 PMID: 6364042
  28. Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments.
    J Bacteriol. 1984 Aug;159(2):783-6 PMID: 6086588
  29. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.14.6327
Published
1988-07-25
Pages
6327-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338298
Subset
IM
Databases
GENBANK
X07465
External Links
PubMed source DOI: 10.1093/nar/16.14.6327 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2