Home LiteratureArticle Details
PMID: 2836697 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell division inhibition gene dicB is regulated by a locus similar to lambdoid bacteriophage immunity loci.

Molecular & general genetics : MGG ·Vol. 212 ·No. 1 ·1988-04-00 ·Pages 11-9

Béjar S, Bouché F, Bouché JP

Abstract

A mutation (dicA1) of a repressor gene located in the terminus region of the Escherichia coli chromosome has previously been shown to lead to temperature-dependent inhibition of division, and to be complemented by plasmids carrying either dicA or an adjacent gene dicC. In this study, operon fusions in the region coding for the division inhibition gene dicB have been used to show that temperature sensitivity does not result from high temperature inactivation of the dicA repressor. Sequence comparisons indicate that dicA and dicC are similar to genes c2 and cro respectively of bacteriophage P22, and carry similarly organized tandem operators, indicating a common evolutionary origin for dicAC and P22 immC. Nevertheless, the consensus half-operator sequence of dicAC, TGTTA-GYYA, differs significantly from that of P22 immC (ATT-TAAGAN). An analysis of the in vivo control of promoters dicAp, dicBp and dicCp placed upstream of malQ shows that the dicAC system is functionally similar to that of an immunity region, with the possible exception of an absence of pairwise cooperative binding. Our results also indicate that the dicA1 mutation causes a switch to permanent control by dicC at all temperatures.

MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence DNA Restriction Enzymes Escherichia coli/genetics,growth & development Genes Genes, Bacterial Genetic Complementation Test Genetic Vectors Genotype Molecular Sequence Data Plasmids Temperature beta-Galactosidase/genetics
Chemicals
DNA Restriction Enzymes beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Béjar S
Centre de Recherches de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
Bouché F
Bouché J P
References (37)
37 references, click to expand
  1. lambda Repressor and cro--components of an efficient molecular switch.
    Nature. 1981 Nov 19;294(5838):217-23 PMID: 6457992
  2. E. coli integration host factor binds to specific sites in DNA.
    Cell. 1984 Dec;39(3 Pt 2):707-16 PMID: 6096022
  3. Primary structure of the phage P22 repressor and its gene c2.
    Biochemistry. 1981 Jun 9;20(12):3591-8 PMID: 7260059
  4. The phi 80 and P22 attachment sites. Primary structure and interaction with Escherichia coli integration host factor.
    J Biol Chem. 1985 Apr 10;260(7):4468-77 PMID: 2984205
  5. Excision and reintegration of the Escherichia coli K-12 chromosomal element e14.
    J Bacteriol. 1985 Mar;161(3):1112-7 PMID: 2982786
  6. Cleavage of the lambda and P22 repressors by recA protein.
    J Biol Chem. 1982 Apr 25;257(8):4458-62 PMID: 6461657
  7. Zygotic induction of the rac locus can cause cell death in E. coli.
    Mol Gen Genet. 1982;187(2):231-5 PMID: 6217397
  8. The operator-binding domain of lambda repressor: structure and DNA recognition.
    Nature. 1982 Jul 29;298(5873):443-7 PMID: 7088190
  9. Essential and nonessential sequences in malPp, a positively controlled promoter in Escherichia coli.
    J Bacteriol. 1985 Mar;161(3):1201-8 PMID: 3156124
  10. Use of statistical criteria for screening potential homologies in nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):203-13 PMID: 6694901
  11. Operator sequences of bacteriophages P22 and 21.
    J Mol Biol. 1980 Feb 15;137(1):81-91 PMID: 6445008
  12. Control of cell division in Escherichia coli. DNA sequence of dicA and of a second gene complementing mutation dicA1, dicC.
    Nucleic Acids Res. 1986 Sep 11;14(17):6821-33 PMID: 3532030
  13. Escherichia coli integration host factor binds specifically to the ends of the insertion sequence IS1 and to its major insertion hot-spot in pBR322.
    J Mol Biol. 1987 May 20;195(2):261-72 PMID: 2821273
  14. Positive control of transcription initiation in bacteria.
    Annu Rev Genet. 1984;18:173-206 PMID: 6084980
  15. Cocrystals of the DNA-binding domain of phage 434 repressor and a synthetic phage 434 operator.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1307-11 PMID: 6369323
  16. Conservation of genome form but not sequence in the transcription antitermination determinants of bacteriophages lambda, phi 21 and P22.
    J Mol Biol. 1985 Jan 5;181(1):75-84 PMID: 3157001
  17. A technique for integrating any DNA fragment into the chromosome of Escherichia coli.
    Gene. 1984 Jul-Aug;29(1-2):231-41 PMID: 6092225
  18. Mechanism of action of the cro protein of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1783-7 PMID: 273909
  19. Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5816-20 PMID: 6091110
  20. Control of transcription by the bacteriophage P22 repressor.
    J Mol Biol. 1982 May 5;157(1):21-48 PMID: 6809952
  21. A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli.
    Mol Gen Genet. 1985;201(2):146-50 PMID: 3911021
  22. Deletion of the terminus region (340 kilobase pairs of DNA) from the chromosome of Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3766-70 PMID: 3889914
  23. Repressor structure and the mechanism of positive control.
    Cell. 1983 Feb;32(2):319-25 PMID: 6218886
  24. Bacteriophage P22 Cro protein: sequence, purification, and properties.
    Biochemistry. 1986 Jan 14;25(1):251-6 PMID: 3954988
  25. SOS-associated division inhibition gene sfiC is part of excisable element e14 in Escherichia coli.
    J Bacteriol. 1986 Oct;168(1):464-6 PMID: 3531184
  26. Restoration by the rac locus of recombinant forming ability in recB - and recC - merozygotes of Escherichia coli K-12.
    Mol Gen Genet. 1973 Apr 12;122(2):119-30 PMID: 4573863
  27. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.
    Gene. 1984 Nov;31(1-3):165-71 PMID: 6098521
  28. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  29. Gene regulation at the right operator (OR) of bacteriophage lambda. II. OR1, OR2, and OR3: their roles in mediating the effects of repressor and cro.
    J Mol Biol. 1980 May 15;139(2):163-94 PMID: 6447795
  30. "N" transcription antitermination proteins of bacteriophages lambda, phi 21 and P22.
    J Mol Biol. 1985 Jan 5;181(1):85-91 PMID: 3157002
  31. A phage repressor-operator complex at 7 A resolution.
    Nature. 1985 Aug 15-21;316(6029):596-601 PMID: 4033757
  32. A convenient technique to compare the efficiency of promoters in Escherichia coli.
    Nucleic Acids Res. 1985 Aug 26;13(16):5919-26 PMID: 3162159
  33. Sequence analysis of a region from the early right operon in phage P22 including the replication genes 18 and 12.
    Gene. 1984 Dec;32(3):289-303 PMID: 6241581
  34. The lambda repressor contains two domains.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1608-12 PMID: 287002
  35. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  36. Homology among DNA-binding proteins suggests use of a conserved super-secondary structure.
    Nature. 1982 Jul 29;298(5873):447-51 PMID: 6896364
  37. Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene.
    EMBO J. 1985 Apr;4(4):1059-66 PMID: 2990907
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00322439
Published
1988-04-00
Pages
11-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00322439
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2