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PMID: 3040670 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of dnaA nucleotide sequences of Escherichia coli, Salmonella typhimurium, and Serratia marcescens.

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 3976-81

Skovgaard O, Hansen FG

Abstract

The dnaA genes of Salmonella typhimurium and Serratia marcescens, which complemented the temperature-sensitive dnaA46 mutation of Escherichia coli, were cloned and sequenced. They were very homologous to the dnaA gene of E. coli. The 63 N-terminal amino acids and the 333 C-terminal amino acids of the corresponding DnaA proteins were identical. The region in between, corresponding to 71 amino acids in E. coli, exhibited a number of changes. This variable region coincided with a nonhomologous region found in the comparison of E. coli dnaA and Bacillus subtilis "dnaA" genes. The regions upstream of the genes were also homologous. The ribosome-binding area, one of the promoters, the DnaA protein-binding site, and many GATC sites (Dam methyltransferase-recognition sequence) were conserved in these three enteric bacteria.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA Replication DNA Restriction Enzymes DNA, Bacterial/analysis Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Genetic Vectors Nucleic Acid Hybridization Plasmids Promoter Regions, Genetic Salmonella typhimurium/genetics Sequence Homology, Nucleic Acid Serratia marcescens/genetics
Chemicals
Bacterial Proteins DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Skovgaard O
Hansen F G
References (35)
35 references, click to expand
  1. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  2. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):979-83 PMID: 165497
  3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  4. Regulation of the dnaA product in Escherichia coli.
    Mol Gen Genet. 1977 Oct 20;155(2):219-25 PMID: 337131
  5. A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature.
    Mol Gen Genet. 1978 Jun 1;162(1):9-16 PMID: 353526
  6. Studies on the regulation of initiation of chromosome replication in Escherichia coli.
    J Mol Biol. 1978 Jul 5;122(3):271-86 PMID: 357730
  7. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  8. Control of the initiation of DNA replication in Escherichia coli. I. Negative control of initiation.
    Mol Gen Genet. 1979 Jan 10;168(2):185-95 PMID: 377010
  9. Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5244-8 PMID: 388433
  10. Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna.
    Mol Gen Genet. 1979 Sep;175(2):135-44 PMID: 160000
  11. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  12. DNA sequence of the Serratia marcescens lipoprotein gene.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1369-73 PMID: 6990409
  13. Chloramphenicol releases a block in initiation of chromosome replication in a dnaA strain of Escherichia coli K12.
    Mol Gen Genet. 1980;178(2):329-36 PMID: 6156379
  14. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  15. Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium.
    J Mol Biol. 1980 Oct 5;142(4):489-502 PMID: 7007651
  16. The initiation of chromosome replication in a dnaAts46 and a dnaA+ strain at various temperatures.
    Mol Gen Genet. 1981;182(2):364-6 PMID: 7026978
  17. Interaction of the Escherichia coli dnaA initiation protein with the dnaZ polymerization protein in vivo.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3340-4 PMID: 6285347
  18. Promoters of the atp operon coding for the membrane-bound ATP synthase of Escherichia coli mapped by Tn10 insertion mutations.
    Mol Gen Genet. 1982;188(2):240-8 PMID: 6185823
  19. The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
    Nucleic Acids Res. 1982 Nov 25;10(22):7373-85 PMID: 6296774
  20. Chromosomal replication origin from the marine bacterium Vibrio harveyi functions in Escherichia coli: oriC consensus sequence.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1164-8 PMID: 6338499
  21. The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli.
    EMBO J. 1982;1(9):1043-8 PMID: 6329723
  22. Structural analysis of the dnaA and dnaN genes of Escherichia coli.
    Gene. 1984 May;28(2):159-70 PMID: 6234204
  23. Functional interchangeability of DNA replication genes in Salmonella typhimurium and Escherichia coli demonstrated by a general complementation procedure.
    Genetics. 1984 Sep;108(1):1-23 PMID: 6237026
  24. The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites.
    Cell. 1984 Oct;38(3):889-900 PMID: 6091903
  25. Allele-specific suppression of dnaA(Ts) mutations by rpoB mutations in Escherichia coli.
    Mol Gen Genet. 1984;197(1):125-8 PMID: 6096668
  26. Autoregulation of the DNA replication gene dnaA in E. coli K-12.
    Cell. 1985 Jan;40(1):159-69 PMID: 2981626
  27. Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region.
    Nucleic Acids Res. 1985 Apr 11;13(7):2251-65 PMID: 2987847
  28. Autoregulation of the dnaA gene of Escherichia coli K12.
    Mol Gen Genet. 1985;200(3):442-50 PMID: 2995766
  29. Conservation of genes and their organization in the chromosomal replication origin region of Bacillus subtilis and Escherichia coli.
    EMBO J. 1985 Dec 1;4(12):3345-50 PMID: 3004954
  30. DNA methylation differentially enhances the expression of one of the two E. coli dnaA promoters in vivo and in vitro.
    Mol Gen Genet. 1986 Feb;202(2):246-50 PMID: 3010047
  31. Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes.
    Mol Gen Genet. 1986 Mar;202(3):435-45 PMID: 3012269
  32. A DNA fragment containing the groE genes can suppress mutations in the Escherichia coli dnaA gene.
    Mol Gen Genet. 1986 Mar;202(3):446-54 PMID: 3012270
  33. crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli.
    J Bacteriol. 1986 Aug;167(2):639-46 PMID: 3525518
  34. Regulation of transcription of the chromosomal dnaA gene of Escherichia coli.
    Mol Gen Genet. 1986 Oct;205(1):115-21 PMID: 3025553
  35. Overproduction of DnaA protein stimulates initiation of chromosome and minichromosome replication in Escherichia coli.
    Mol Gen Genet. 1987 Jan;206(1):51-9 PMID: 3033441
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.9.3976-3981.1987
Published
1987-09-00
Pages
3976-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213696
Subset
IM
Databases
GENBANK
M17351, M17352, M17353
External Links
PubMed source DOI: 10.1128/jb.169.9.3976-3981.1987 PMC Full Text View journal details
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