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PMID: 3025553 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of transcription of the chromosomal dnaA gene of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 205 ·No. 1 ·1986-10-00 ·Pages 115-21

Kücherer C, Lother H, Kölling R, Schauzu MA, Messer W

Abstract

By comparative S1 analysis we investigated the in vivo regulation of transcription of the chromosomal dnaA gene coding for a protein essential for the initiation of replication at the chromosomal origin. Inactivation of the protein in dnaA mutants results in derepression, whereas excess DnaA protein (presence of a DnaA overproducing plasmid) leads to repression of dnaA transcription. Both dnaA promoters are subject to autoregulation allowing modulation of transcriptional efficiency by at least 20-fold. Increasing the number of oriC sequences (number of DnaA binding sites) in the cell by introducing oriC plasmids leads to a derepression of transcription. Autoregulation and binding to oriC suggest that the DnaA protein exerts a major role in the regulation of the frequency of initiation at oriC. The efficiency of transcription of the dnaA2 promoter is reduced in the absence of dam methylation, which is involved in the regulation of oriC replication.

MeSH Terms
Bacterial Proteins/genetics Chromosomes, Bacterial/physiology Escherichia coli/genetics Genes Genes, Bacterial Genes, Regulator Genotype Mutation Promoter Regions, Genetic Transcription, Genetic
Chemicals
Bacterial Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kücherer C
Lother H
Kölling R
Schauzu M A
Messer W
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51 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02428040
Published
1986-10-00
Pages
115-21
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02428040
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