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PMID: 3039349 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rapid and selective alterations in the expression of cellular genes accompany conditional transcription of Ha-v-ras in NIH 3T3 cells.

Molecular and cellular biology ·Vol. 7 ·No. 7 ·1987-07-00 ·Pages 2512-20

Owen RD, Ostrowski MC

Abstract

Hormone treatment of NIH 3T3 cells that contain recombinant fusions between the mouse mammary virus long terminal repeat and the v-ras gene of Harvey murine sarcoma virus results in conditional expression of the ras p21 gene product. Levels of ras mRNA and p21 are maximal after 2 to 4 h of hormone treatment. Analysis of cellular RNA by Northern blotting and nuclease S1 protection assays indicates that the expression of two cellular RNA species increases with kinetics similar to v-ras: v-sis-related RNA and retrovirus-related VL30 RNA. Run-on transcription in isolated nuclei shows that the increase in v-sis-related RNA is not dependent on transcription and therefore must arise by a post-transcriptional mechanism. The increase in VL30 expression is a transcriptional effect. Hormone treatment of normal NIH 3T3 cells has no effect on the expression of these DNA sequences. These results suggest that v-ras stimulation of autocrine factors may play a role in transformation of cells by this gene and also suggest a reverse genetic strategy to determine the nucleic acid sequences and cellular factors involved in the regulation of gene expression that is observed.

MeSH Terms
Animals Cell Line Gene Expression Regulation Harvey murine sarcoma virus/genetics Mammary Tumor Virus, Mouse/genetics Oncogenes Proto-Oncogene Proteins/biosynthesis,genetics Proto-Oncogene Proteins p21(ras) RNA, Messenger/genetics,metabolism Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Proto-Oncogene Proteins RNA, Messenger Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Owen R D
Ostrowski M C
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.7.2512-2520.1987
Published
1987-07-00
Pages
2512-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365385
Subset
IM
Grants
NIGMS NIH HHS · GM 34615-03 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.7.2512-2520.1987 PMC Full Text View journal details
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