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PMID: 3037546 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 15 ·1987-08-00 ·Pages 5364-8

Saga Y, Tung JS, Shen FW, Boyse EA

Abstract

Previous inferences that Ly-5 glycoprotein isoforms of murine hematopoietic cells are generated by alternative splicing of primary transcripts of a single Ly-5 gene are supported by the present study. A cDNA library was prepared from B cells by extension from primer representing a known T-cell cDNA sequence. Three different Ly-5 clones from this library included sequences missing in T-cell cDNA clones. From the constitution of cDNA clones and of the Ly-5 gene, and from S1 nuclease mapping, it is concluded that at least two exons, provisionally numbered Ex-6(B) and Ex-7(B), in the 5'-proximal region are mainly represented in mRNA of the B-cell lines examined but not of the T-cell lines examined. Also, exons 1 and 2 appear to be used alternatively in different species of B-cell mRNA and probably also in different species of T-cell mRNA.

MeSH Terms
Animals Base Sequence DNA/analysis Endonucleases/metabolism Hematopoiesis Histocompatibility Antigens/genetics Leukocyte Common Antigens Mice RNA Splicing Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Histocompatibility Antigens DNA Endonucleases Leukocyte Common Antigens Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Saga Y
Tung J S
Shen F W
Boyse E A
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.15.5364
Published
1987-08-00
Pages
5364-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298856
Subset
IM
Grants
NCI NIH HHS · P30 CA008748 · United States
NCI NIH HHS · CA-39827 · United States
NIAID NIH HHS · AI-21840 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.15.5364 PMC Full Text View journal details
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