Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes.

Molecular and cellular biology ·Vol. 7 ·No. 6 ·1987-06-00 ·Pages 2097-103

Giorda R, Ennis HL

Abstract

A previously isolated cDNA clone, pLK229, that is specific for mRNA developmentally expressed during Dictyostelium discoideum spore germination and multicellular development, was used to screen two genomic libraries. Two genomic sequences homologous to pLK229 were isolated and sequenced. Genomic clone p229 is identical to the cDNA clone pLK229 and codes for a polypeptide of 381 amino acids. This polypeptide is composed of five tandem repeats of the same 76-amino-acid sequence. Clone lambda 229 codes for a protein of 229 amino acids, containing three tandem repeats of the identical 76-amino-acid sequence. A computer search for homology to known proteins revealed that the 76-amino-acid repeat was identical to human and bovine ubiquitin except for two amino acid differences.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Dictyostelium/genetics Genes Genes, Fungal RNA, Messenger/genetics Transcription, Genetic Ubiquitins/genetics

Chemicals

RNA, Messenger Ubiquitins DNA DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Giorda R

Ennis H L

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30 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.7.6.2097-2103.1987

Published

1987-06-00

Pages

2097-103

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC365330

Subset

External Links

PubMed source DOI: 10.1128/mcb.7.6.2097-2103.1987 PMC Full Text View journal details

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Structure of two developmentally regulated Dictyostelium discoideum ubiquitin genes.

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