Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 2031-4

Brash DE, Reddel RR, Quanrud M, Yang K, Farrell MP, Harris CC

Abstract

Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).

MeSH Terms

Antigens, Viral, Tumor/genetics Bronchi/cytology,physiology Cells, Cultured Chemical Precipitation Epithelial Cells Gene Expression Regulation Genes, Viral Humans Phosphates Simian virus 40 Strontium Transfection

Chemicals

Antigens, Viral, Tumor Phosphates strontium phosphate Strontium

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Brash D E

Reddel R R

Quanrud M

Yang K

Farrell M P

Harris C C

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26 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.7.5.2031-2034.1987

Published

1987-05-00

Pages

2031-4

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC365315

Subset

External Links

PubMed source DOI: 10.1128/mcb.7.5.2031-2034.1987 PMC Full Text View journal details

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Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells.

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