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PMID: 3036062 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fluoroaluminates mimic guanosine 5'-[gamma-thio]triphosphate in activating the polyphosphoinositide phosphodiesterase of hepatocyte membranes. Role for the guanine nucleotide regulatory protein Gp in signal transduction.

The Biochemical journal ·Vol. 241 ·No. 2 ·1987-01-15 ·Pages 409-14

Cockcroft S, Taylor JA

Abstract

Fluoride and guanosine 5'-[gamma-thio]triphosphate (GTP gamma S) both activate the hepatocyte membrane polyphosphoinositide phosphodiesterase (PPI-pde) in a concentration-dependent manner. AlCl3 enhances the fluoride effect, supporting the concept that [A1F4]- is the active species. Analysis of the products of inositol lipid hydrolysis demonstrate that phosphatidylinositol bisphosphate is the major lipid to be hydrolysed. Guanosine 5'-[beta-thio]diphosphate (GDP beta S) is an inhibitor of activation of PPI-pde by both fluoride and GTP gamma S. These observations suggest that the guanine nucleotide regulatory protein (termed Gp) bears a structural resemblance to the well-characterized G-proteins of the adenylate cyclase system and the cyclic GMP phosphodiesterase system in phototransduction.

MeSH Terms
Aluminum/pharmacology Aluminum Chloride Aluminum Compounds Animals Cell Membrane/drug effects,enzymology Chlorides/pharmacology Enzyme Activation/drug effects GTP-Binding Proteins/metabolism Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate/analogs & derivatives,pharmacology In Vitro Techniques Liver/drug effects,enzymology Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases/metabolism Rats Sodium Fluoride/pharmacology Thionucleotides/pharmacology
Chemicals
Aluminum Compounds Chlorides Thionucleotides Guanosine 5'-O-(3-Thiotriphosphate) Aluminum Chloride Guanosine Triphosphate Sodium Fluoride Aluminum Phosphoric Diester Hydrolases Phosphoinositide Phospholipase C GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cockcroft S
Taylor J A
References (41)
41 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2410409
Published
1987-01-15
Pages
409-14
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147575
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2410409 PMC Full Text View journal details
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