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PMID: 3036042 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Electropherotype heterogeneity within serotypes of human rotavirus strains circulating in Italy. Brief report.

Archives of virology ·Vol. 95 ·No. 1-2 ·1987-00-00 ·Pages 129-35

Gerna G, Arista S, Passarani N, Sarasini A, Battaglia M

Abstract

Using solid-phase immune electron microscopy, 126 of 129 human rotavirus (HRV) strains could be serotyped directly in stools collected in Italy during the period 1981-1985. Prevalence was 70.5 per cent for serotype 1, and 13.2 per cent for each of serotypes 2 and 4. No serotype 3 strain was detected. In parallel, for 39 of 61 HRV strains tested the electropherotype of genomic RNA was successfully determined. Different electropherotypes were detected among strains of the same serotype, whereas the same electropherotype was found in HRV strains of different serotypes. Serotyping and electropherotyping of HRV strains appear to be complementary to each other, and both should be used in conjunction for epidemiological surveys.

MeSH Terms
Child, Preschool Electrophoresis, Polyacrylamide Gel Feces/microbiology Humans Infant Italy RNA, Viral/analysis Rotavirus/classification,isolation & purification Serotyping
Chemicals
RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gerna G
Arista S
Passarani N
Sarasini A
Battaglia M
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18 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01311340
Published
1987-00-00
Pages
129-35
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01311340 View journal details
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