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PMID: 3035563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Multiple genes encode the human Na+,K+-ATPase catalytic subunit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 12 ·1987-06-00 ·Pages 4039-43

Shull MM, Lingrel JB

Abstract

A human genomic library was constructed and screened with hybridization probes derived from sheep and rat cDNAs encoding the alpha and alpha(+) isoforms, respectively, of the Na+,K+-ATPase catalytic subunit. Genomic sequences spanning 150 kilobases were isolated. Four genes, designated alpha A, alpha B, alpha C, and alpha D, each 20-25 kilobases in length, were identified by restriction mapping, Southern blot hybridization analysis, and limited DNA sequencing. We present evidence that two of these genes, alpha A and alpha B, encode the alpha and alpha(+) isoforms, respectively. The other genes, alpha C and alpha D, one of which is physically linked to the alpha(+) gene, exhibit nucleotide and amino acid homology to Na+,K+-ATPase catalytic subunit cDNA sequences but do not correspond to any previously identified isoforms.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes Humans Kidney/enzymology Leukocytes/enzymology Macromolecular Substances Nucleic Acid Hybridization Sheep Sodium-Potassium-Exchanging ATPase/blood,genetics
Chemicals
Macromolecular Substances DNA DNA Restriction Enzymes Sodium-Potassium-Exchanging ATPase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shull M M
Lingrel J B
References (34)
34 references, click to expand
  1. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  2. Dependence of renal (Na+ + k+)-adenosine triphosphatase activity on thyroid status.
    J Biol Chem. 1976 Dec 25;251(24):7826-33 PMID: 137242
  3. Increased (Na+,K+)-ATPase concentrations in various tissues of rats caused by thyroid hormone treatment.
    J Biol Chem. 1978 Feb 10;253(3):723-6 PMID: 146037
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Two molecular forms of (Na+ + K+)-stimulated ATPase in brain. Separation, and difference in affinity for strophanthidin.
    J Biol Chem. 1979 Jul 10;254(13):6060-7 PMID: 221488
  6. Mineralocorticoid effects on Na-K-ATPase in individual nephron segments.
    Am J Physiol. 1981 Jun;240(6):F536-44 PMID: 6264796
  7. Polymorphic DNA region adjacent to the 5' end of the human insulin gene.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5759-63 PMID: 6272317
  8. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  9. Ontogeny of the (Na+,K+)-ATPase during chick skeletal myogenesis.
    J Biol Chem. 1982 May 25;257(10):5380-4 PMID: 6279598
  10. Hormonal regulation of (Na+,K+)-ATPase biosynthesis in the toad bladder. Effect of aldosterone and 3,5,3'-triiodo-L-thyronine.
    J Biol Chem. 1982 Sep 10;257(17):10338-43 PMID: 6286651
  11. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  12. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  13. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  14. Specific inactivation of alpha (+) molecular form of (Na+ + K+)-ATPase by pyrithiamin.
    J Biol Chem. 1984 Mar 25;259(6):3858-63 PMID: 6323444
  15. Effects of growth in low potassium medium or ouabain on membrane Na,K-ATPase, cation transport, and contractility in cultured chick heart cells.
    Circ Res. 1984 Jul;55(1):39-48 PMID: 6086172
  16. Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7417-20 PMID: 6209716
  17. Identification of two molecular forms of (Na+,K+)-ATPase in rat adipocytes. Relation to insulin stimulation of the enzyme.
    J Biol Chem. 1985 Jan 25;260(2):1177-84 PMID: 2981837
  18. Insulin affects the sodium affinity of the rat adipocyte (Na+,K+)-ATPase.
    J Biol Chem. 1985 Aug 25;260(18):10075-80 PMID: 2991273
  19. Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA.
    Nature. 1985 Aug 22-28;316(6030):691-5 PMID: 2993903
  20. Amino-acid sequence of a Ca2+ + Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence.
    Nature. 1985 Aug 22-28;316(6030):696-700 PMID: 2993904
  21. Primary structure of the alpha-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequence.
    Nature. 1985 Aug 22-28;316(6030):733-6 PMID: 2993905
  22. Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6357-61 PMID: 2994074
  23. Two Ca2+ ATPase genes: homologies and mechanistic implications of deduced amino acid sequences.
    Cell. 1986 Feb 28;44(4):597-607 PMID: 2936465
  24. Na+-ATPase is a different entity from the (Na+ + K+)-ATPase in rat kidney basolateral plasma membranes.
    Biochim Biophys Acta. 1986 Jun 13;858(1):202-5 PMID: 3011092
  25. Pig kidney Na+,K+-ATPase. Primary structure and spatial organization.
    FEBS Lett. 1986 Jun 9;201(2):237-45 PMID: 2423371
  26. Blood--brain barrier sodium/potassium pump: modulation by central noradrenergic innervation.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):4067-70 PMID: 3012548
  27. Regulation of the (Na+ + K+)-ATPase in cultured chick skeletal muscle. Modulation of expression by the demand for ion transport.
    J Biol Chem. 1986 Jul 25;261(21):9990-9 PMID: 3015915
  28. Developmental and thyroid hormone regulation of two molecular forms of Na+-K+-ATPase in brain.
    J Biol Chem. 1986 Aug 5;261(22):10439-44 PMID: 3015931
  29. Primary structure of the alpha-subunit of human Na,K-ATPase deduced from cDNA sequence.
    J Biochem. 1986 Aug;100(2):389-97 PMID: 2430951
  30. Molecular cloning of the rat stomach (H+ + K+)-ATPase.
    J Biol Chem. 1986 Dec 25;261(36):16788-91 PMID: 3023364
  31. Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.
    Biochemistry. 1986 Dec 16;25(25):8125-32 PMID: 3028470
  32. Effect of thyroid hormone on the abundance of Na,K-adenosine triphosphatase alpha-subunit messenger ribonucleic acid.
    Mol Endocrinol. 1987 Jan;1(1):83-9 PMID: 2842662
  33. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  34. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.12.4039
Published
1987-06-00
Pages
4039-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305017
Subset
IM
Grants
NHLBI NIH HHS · HL28573 · United States
Databases
GENBANK
M16793, M16794, M16795, M16796, M16797, M16798
External Links
PubMed source DOI: 10.1073/pnas.84.12.4039 PMC Full Text View journal details
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