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PMID: 3029726 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis.

Nucleic acids research ·Vol. 15 ·No. 4 ·1987-02-25 ·Pages 1753-61

Neale AD, Scopes RK, Wettenhall RE, Hoogenraad NJ

Abstract

Pyruvate decarboxylase (EC 4.1.1.1), the penultimate enzyme in the alcoholic fermentation pathway of Zymomonas mobilis, converts pyruvate to acetaldehyde and carbon dioxide. The complete nucleotide sequence of the structural gene encoding pyruvate decarboxylase from Zymomonas mobilis has been determined. The coding region is 1704 nucleotides long and encodes a polypeptide of 567 amino acids with a calculated subunit mass of 60,790 daltons. The amino acid sequence was confirmed by comparison with the amino acid sequence of a selection of tryptic fragments of the enzyme. The amino acid composition obtained from the nucleotide sequence is in good agreement with that obtained experimentally.

MeSH Terms
Amino Acid Sequence Base Sequence Carboxy-Lyases/genetics Codon DNA Restriction Enzymes Genes Genes, Bacterial Gram-Negative Bacteria/enzymology,genetics Pyruvate Decarboxylase/genetics
Chemicals
Codon DNA Restriction Enzymes Carboxy-Lyases Pyruvate Decarboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Neale A D
Scopes R K
Wettenhall R E
Hoogenraad N J
References (9)
9 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.4.1753
Published
1987-02-25
Pages
1753-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340579
Subset
IM
Databases
GENBANK
M15368
External Links
PubMed source DOI: 10.1093/nar/15.4.1753 PMC Full Text View journal details
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