Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

Nucleic acids research ·Vol. 15 ·No. 1 ·1987-01-12 ·Pages 219-32

Ono A, Ueda T

Abstract

The naturally-occurring modified bases, N6-methyladenine, N4-methylcytosine, and 5-methylcytosine were chemically introduced in place of the adenine or cytosine in the decadeoxyribonucleotides containing recognition sequences of Bgl II, Sau 3AI, Mbo I and Mfl I. The modified oligomers bind to the enzymes but the rates of cleavage by the enzymes are variable.

MeSH Terms

5-Methylcytosine Adenine/analogs & derivatives Circular Dichroism Cytosine/analogs & derivatives DNA Restriction Enzymes/metabolism Indicators and Reagents Kinetics Nucleic Acid Conformation Nucleic Acid Denaturation Oligodeoxyribonucleotides/chemical synthesis Substrate Specificity

Chemicals

Indicators and Reagents Oligodeoxyribonucleotides N(4)-methylcytosine 5-Methylcytosine Cytosine DNA Restriction Enzymes Adenine 6-methyladenine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ono A

Ueda T

References (26)

26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/15.1.219

Published

1987-01-12

Pages

219-32

Language

English

Region

England

NLM ID

0411011

PMCID

PMC340406

Subset

External Links

PubMed source DOI: 10.1093/nar/15.1.219 PMC Full Text View journal details

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Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

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