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PMID: 3027666 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Methylated DNA-binding protein from human placenta recognizes specific methylated sites on several prokaryotic DNAs.

Nucleic acids research ·Vol. 14 ·No. 24 ·1986-12-22 ·Pages 9843-60

Wang RY, Zhang XY, Khan R, Zhou YW, Huang LH, Ehrlich M

Abstract

Methylated DNA-binding protein (MDBP) from human placenta recognizes specific DNA sequences containing 5-methylcytosine (m5C) residues. Comparisons of binding of various prokaryotic DNAs to MDBP indicate that m5CpG is present in the recognition sites for this protein but is only part of the recognition sequence. Specific binding to MDBP was observed for bacteriophage XP12 DNA, which naturally contains approximately 1/3 of its residues as m5C, and for Micrococcus luteus DNA, M13mp8 replicative form (RF) DNA, and pBR322 when these three DNAs were methylated at CpG sites by human DNA methyltransferase. Five DNA regions binding to MDBP have been localized by DNase I footprinting or restriction mapping in methylated pBR322 and M13mp8 RF DNAs. A comparison of their sequences reveals a common 5'-m5CGRm5CG-3' element or closely related sequence in which one of the m5C residues may be replaced by a T. In addition to this motif, one upstream and one downstream m5CpG as well as other common residues over an approximately 20-bp long region may be recognized by MDBP.

MeSH Terms
Base Sequence DNA/metabolism DNA Restriction Enzymes DNA-Binding Proteins/isolation & purification,metabolism Female Humans Methylation Placenta/metabolism Pregnancy Proteins/metabolism Protein Binding Structure-Activity Relationship Substrate Specificity
Chemicals
DNA-Binding Proteins Pregnancy Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wang R Y
Zhang X Y
Khan R
Zhou Y W
Huang L H
Ehrlich M
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45 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.24.9843
Published
1986-12-22
Pages
9843-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341339
Subset
IM
Grants
NIGMS NIH HHS · GM33999 · United States
External Links
PubMed source DOI: 10.1093/nar/14.24.9843 PMC Full Text View journal details
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