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PMID: 3027411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of nonpermissivity in mouse cells overexpressing simian virus 40 T antigen.

Journal of virology ·Vol. 61 ·No. 3 ·1987-03-00 ·Pages 851-7

Gerard RD, Guggenheimer RA, Gluzman Y

Abstract

To analyze the nature of the nonpermissivity of mouse cells for simian virus 40 (SV40) DNA replication, we isolated mouse cells producing SV40 T antigen (Tag) at levels equal to or greater than that found in COS1 cells. These mouse cells were nonpermissive for the replication of exogenously added SV40 DNA, although purified Tag isolated from these cells was able to support SV40 DNA replication in vitro. Furthermore, when mouse cells expressing Tag were fused to monkey cells, SV40 DNA replication was observed. These results indicate that the mere production of large quantities of wild-type SV40 Tag does not overcome the block of nonpermissivity in mouse cells and that cellular factors must play a critical role.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics Cell Line Cloning, Molecular DNA Replication DNA, Viral/biosynthesis Gene Expression Regulation Mice Simian virus 40/genetics,growth & development Species Specificity Transformation, Genetic Virus Replication
Chemicals
Antigens, Viral, Tumor DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gerard R D
Guggenheimer R A
Gluzman Y
References (30)
30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.3.851-857.1987
Published
1987-03-00
Pages
851-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254029
Subset
IM
Grants
NCI NIH HHS · CA-07493 · United States
NCI NIH HHS · CA-13106 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.3.851-857.1987 PMC Full Text View journal details
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