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PMID: 3025812 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Comparison of promoter suppression in avian and murine retrovirus vectors.

Nucleic acids research ·Vol. 14 ·No. 23 ·1986-12-09 ·Pages 9381-96

Emerman M, Temin HM

Abstract

Previously, we described "promoter suppression" in infectious retrovirus vectors with two genes and an internal promoter. Here, we examined several parameters of promoter suppression and found that the amount of suppression in an integrated retrovirus vector was dependent both on whether the vector was derived from spleen necrosis virus or murine leukemia virus and on which internal promoter was present in the vector. Murine leukemia virus vectors showed less suppression than analogous spleen necrosis virus vectors. Furthermore, the amount of suppression was not dependent on either the relative strengths of the promoters nor the distance between the promoters. Moreover, we found that in vectors in which one promoter was suppressed, there was an inverse correlation between the DNaseI sensitivity of the chromatin surrounding a promoter and the suppression of its expression.

MeSH Terms
Chromatin/metabolism Deoxyribonuclease I/pharmacology Genetic Vectors Leukemia Virus, Murine/genetics Neomycin/pharmacology Phenotype Promoter Regions, Genetic R Factors Repetitive Sequences, Nucleic Acid Retroviridae/genetics Suppression, Genetic Thymidine Kinase/genetics Transcription, Genetic Transfection
Chemicals
Chromatin Thymidine Kinase Deoxyribonuclease I Neomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Emerman M
Temin H M
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.23.9381
Published
1986-12-09
Pages
9381-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311965
Subset
IM
Grants
NCI NIH HHS · CA-07175 · United States
NCI NIH HHS · CA-09135-10 · United States
NCI NIH HHS · CA-22443 · United States
External Links
PubMed source DOI: 10.1093/nar/14.23.9381 PMC Full Text View journal details
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