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PMID: 3025189 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of transposable elements which activate gene expression in Pseudomonas cepacia.

Journal of bacteriology ·Vol. 169 ·No. 1 ·1987-01-00 ·Pages 8-13

Scordilis GE, Ree H, Lessie TG

Abstract

This study demonstrated that transposable elements in Pseudomonas cepacia could be inserted upstream of a poorly expressed gene and increase its expression more than 30-fold. Five elements, TnPc1, IS402, IS403, IS404, and IS405, were isolated by their ability to increase expression of the beta-lactamase gene of the broad-host-range plasmid pRP1. Increased expression resulted only from insertion of these elements, suggesting that insertional activation is an important means of elevating gene expression in this organism. Four of the elements inserted between a PstI site within the beta-lactamase gene and a BamHI site located 375 base pairs upstream of its promoter. The element IS403 inserted distal to the BamHI site within the coding region for the gene tnpR, suggesting that insertional activation can act over greater than expected distances. In addition, the element IS402 activated the beta-lactamase genes carried on plasmids pRP1 and pMR5 (temperature-sensitive pRP1) equally well in opposite orientations, demonstrating that insertional activation by this element occurs independent of its orientation.

MeSH Terms
DNA Restriction Enzymes/metabolism DNA Transposable Elements Gene Expression Regulation Nucleic Acid Hybridization Plasmids Pseudomonas/enzymology,genetics beta-Lactamases/genetics
Chemicals
DNA Transposable Elements DNA Restriction Enzymes beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scordilis G E
Ree H
Lessie T G
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.1.8-13.1987
Published
1987-01-00
Pages
8-13
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211726
Subset
IM
Grants
NIGMS NIH HHS · GM-21198 · United States
External Links
PubMed source DOI: 10.1128/jb.169.1.8-13.1987 PMC Full Text View journal details
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