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PMID: 3021402 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of SV40 chromatin by mass determination on STEM.

Chromosoma ·Vol. 94 ·No. 3 ·1986-00-00 ·Pages 189-98

Schultz P, Weiss E, Colin P, Régnier E, Oudet P

Abstract

Direct mass determination of purified SV40 minichromosomes was obtained by scanning transmission electron microscopy. Twenty to thirty percent of the minichromosomes were found with an Mr of 6.9 +/- 0.4 X 10(6). The rest of the molecules formed a spread Mr distribution ranging from 7.3 X 10(6) to 9.5 X 10(6) due possibly to different contents of the virus-coded proteins, mainly VP1. The apparent mass histogram of individual SV40 nucleosomes presents three maxima at Mr 2.1 X 10(5), 2.6 X 10(5) and 3.1 X 10(5) that could correspond to partially unravelled nucleosomes, complete nucleosomes and complete nucleosomes with the addition of VP1. Beaded structures with a higher mass were also measured; some were found at either side of the open nucleosome-free region.

MeSH Terms
Chromatin/analysis,ultrastructure DNA, Viral/analysis Microscopy, Electron, Scanning Molecular Weight Nucleosomes/ultrastructure Simian virus 40/ultrastructure Viral Proteins/analysis
Chemicals
Chromatin DNA, Viral Nucleosomes Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schultz P
Weiss E
Colin P
Régnier E
Oudet P
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38 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00288493
Published
1986-00-00
Pages
189-98
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00288493 View journal details
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