Home LiteratureArticle Details

PMID: 3010302 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synaptophysin: a marker protein for neuroendocrine cells and neoplasms.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 10 ·1986-05-00 ·Pages 3500-4

Wiedenmann B, Franke WW, Kuhn C, Moll R, Gould VE

Abstract

Synaptophysin is an integral membrane glycoprotein (Mr 38,000) that occurs in presynaptic vesicles of neurons and in similar vesicles of the adrenal medulla. By using a monoclonal antibody to this protein (SY38), we have found, by immunohistochemistry and immunoblotting, that an identical or similar protein is also expressed in neuroendocrine tumors of neural type, such as pheochromocytomas and paragangliomas. In addition, this protein occurs in certain neuroendocrine epithelial cells, such as pancreatic islet cells; in a variety of neuroendocrine epithelial tumors, including isletcell adenomas and carcinomas and several carcinoids and neuroendocrine carcinomas of the gastrointestinal and the bronchial tracts; and in medullary carcinomas of the thyroid. Our results show that synaptophysin, and the vesicles that contain it, can occur in normal and neoplastic neuroendocrine cells of neural type, as demonstrated by colocalization with neurofilaments, as well as in those of epithelial type, as shown by colocalization with cytokeratin filaments and desmoplakins. We conclude that synaptophysin is expressed independently of other neuronal differentiation markers and propose that it be used as a differentiation marker in tumor diagnosis.

MeSH Terms

Adrenal Medulla/metabolism Antibodies, Monoclonal Endocrine Glands/metabolism Epithelium/metabolism Fluorescent Antibody Technique Humans Immunosorbent Techniques Islets of Langerhans/metabolism Membrane Proteins/metabolism Neoplasm Proteins/metabolism Neoplasms, Glandular and Epithelial/metabolism Neoplasms, Nerve Tissue/metabolism Nerve Tissue Proteins/metabolism Synaptic Vesicles/metabolism Synaptophysin Tissue Distribution

Chemicals

Antibodies, Monoclonal Membrane Proteins Neoplasm Proteins Nerve Tissue Proteins Synaptophysin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Wiedenmann B

Franke W W

Kuhn C

Moll R

Gould V E

References (30)

30 references, click to expand

Neurone-specific enolase is a molecular marker for peripheral and central neuroendocrine cells.
Nature. 1978 Dec 21-28;276(5690):834-6 PMID: 31568
The cytochemistry and ultrastructure of polypeptide hormone-producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept.
J Histochem Cytochem. 1969 May;17(5):303-13 PMID: 4143745
Viral transformation of chick myogenic cells. The relationship between differentiation and the expression of the SRC gene.
Exp Cell Res. 1979 Oct 1;123(1):95-105 PMID: 226384
Neuron-specific enolase is produced by neuroendocrine tumours.
Lancet. 1981 Apr 11;1(8224):808-11 PMID: 6111674
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
J Histochem Cytochem. 1981 Apr;29(4):577-80 PMID: 6166661
Pancreatic endocrine tumors.
Hum Pathol. 1982 Mar;13(3):263-71 PMID: 7076209
Diversity of cytokeratins. Differentiation specific expression of cytokeratin polypeptides in epithelial cells and tissues.
J Mol Biol. 1981 Dec 25;153(4):933-59 PMID: 6177862
Neuron specific enolase: a common marker for the endocrine cells and innervation of the gut and pancreas.
Gastroenterology. 1982 Oct;83(4):902-15 PMID: 7106520
The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
Cell. 1982 Nov;31(1):11-24 PMID: 6186379
Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.
Lab Invest. 1983 Apr;48(4):372-94 PMID: 6187996
Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins.
Proc Natl Acad Sci U S A. 1983 Jan;80(2):543-7 PMID: 6340102
Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, antiintermediate filament antibodies, and lectins.
Lab Invest. 1983 Sep;49(3):317-26 PMID: 6193332
Similarity of secretory protein I from parathyroid gland to chromogranin A from adrenal medulla.
Proc Natl Acad Sci U S A. 1982 Oct;79(19):6056-9 PMID: 6821132
Neuroendocrine components of the bronchopulmonary tract: hyperplasias, dysplasias, and neoplasms.
Lab Invest. 1983 Nov;49(5):519-37 PMID: 6138458
Monoclonal antibodies specific for glial fibrillary acidic (GFA) protein and for each of the neurofilament triplet polypeptides.
Differentiation. 1983;25(2):193-203 PMID: 6198232
Immunohistochemical and ultrastructural analysis of bronchopulmonary neuroendocrine neoplasms. I. Carcinoids.
Ultrastruct Pathol. 1984;6(1):15-27 PMID: 6375057
Coexpression of neuroendocrine markers and epithelial cytoskeletal proteins in bronchopulmonary neuroendocrine neoplasms.
Lab Invest. 1985 Jan;52(1):39-51 PMID: 2981372
Chromogranin immunoreactivity in the central nervous system. Immunochemical characterisation, distribution and relationship to catecholamine and enkephalin pathways.
Brain Res. 1984 Dec;320(2-3):193-230 PMID: 6084534
Identification of Merkel cells in human skin by specific cytokeratin antibodies: changes of cell density and distribution in fetal and adult plantar epidermis.
Differentiation. 1984;28(2):136-54 PMID: 6084624
Immunohistochemical localization of gamma-enolase in normal human tissues other than nervous and neuroendocrine tissues.
Lab Invest. 1985 Mar;52(3):257-63 PMID: 3974199
Immunofluorescence microscopic evaluation of the intermediate filament expression of the adrenal cortex and medulla and their tumors.
Am J Pathol. 1985 Mar;118(3):360-6 PMID: 3883796
Neuroendocrine (Merkel) cells of the skin: hyperplasias, dysplasias, and neoplasms.
Lab Invest. 1985 Apr;52(4):334-53 PMID: 2580118
Varying expression of cytokeratin and neurofilaments in neuroendocrine tumors of human gastrointestinal tract.
Lab Invest. 1985 Apr;52(4):429-36 PMID: 2580121
A dual expression of cytokeratin and neurofilaments in bronchial carcinoid cells.
Int J Cancer. 1985 Apr 15;35(4):421-5 PMID: 2580799
A 38,000-dalton membrane protein (p38) present in synaptic vesicles.
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4137-41 PMID: 3923488
Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles.
Cell. 1985 Jul;41(3):1017-28 PMID: 3924408
Differential expression of intermediate filament proteins distinguishes classic from variant small-cell lung cancer cell lines.
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4409-13 PMID: 2409557
The complement of desmosomal plaque proteins in different cell types.
J Cell Biol. 1985 Oct;101(4):1442-54 PMID: 2413044
Secretogranins I and II: two tyrosine-sulfated secretory proteins common to a variety of cells secreting peptides by the regulated pathway.
J Cell Biol. 1985 Nov;101(5 Pt 1):1999-2011 PMID: 4055903
Embryology of the diffuse neuroendocrine system and its relationship to the common peptides.
Fed Proc. 1979 Aug;38(9):2288-94 PMID: 37125

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.10.3500

Published

1986-05-00

Pages

3500-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC323544

Subset

External Links

PubMed source DOI: 10.1073/pnas.83.10.3500 PMC Full Text View journal details

Analysis Services

Synaptophysin: a marker protein for neuroendocrine cells and neoplasms.

Contact

WeChat Official Account