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PMID: 3007345 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

HLA-JY328: mapping studies and expression of a polymorphic HLA class I gene.

Immunogenetics ·Vol. 23 ·No. 2 ·1986-00-00 ·Pages 90-9

Duceman BW, Ness D, Rende R, Chorney MJ, Srivastava R, Greenspan DS, Pan J, Weissman SM, Grumet FC

Abstract

The JY328 clone was identified in a human genomic library using cDNA corresponding to mRNA for HLA-B7 as a probe. The L/328 cell line was established by cotransformation of mouse Ltk- cells with the herpes thymidine kinase gene and clone JY328. On Northern blots, RNA from L/328 strongly hybridized to an HLA class I probe, and an antigen was recognized by an anti-HLA class I framework antibody on the cell surface. A DNA probe corresponding to a segment of intron 7 was developed by comparing the nucleotide sequence of clone JY328 with that of other HLA class I-type genes. Using the radiolabeled probe to screen Southern blots of DNA from families with siblings exhibiting intra-HLA recombinations, a restriction fragment length polymorphism was revealed--a 1.4 kb BstE II band not present in all individuals. A corresponding fragment was apparent in the base sequence of clone JY328. The occurrence of this band on Southern blots established that JY328 maps distinct from and centromeric to the HLA-C locus and near to the HLA-B locus. Antibody absorption studies and cytotoxicity tests indicated that the JY328 gene product was not an HLA-B antigen but that it did specifically absorb CW7-specific antibody. In sum, these results suggest a novel, polymorphic HLA class I gene which expresses a product serologically similar to HLA-Cw7 but which does not map within the corresponding locus.

MeSH Terms
Animals Antigens, Surface/analysis B-Lymphocytes/analysis,immunology DNA/analysis,genetics DNA Restriction Enzymes Genetic Markers HLA Antigens/genetics HLA-B27 Antigen Humans L Cells/analysis Major Histocompatibility Complex Mice Polymorphism, Genetic Sequence Homology, Nucleic Acid
Chemicals
Antigens, Surface Genetic Markers HLA Antigens HLA-B27 Antigen DNA DNA Restriction Enzymes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Duceman B W
Ness D
Rende R
Chorney M J
Srivastava R
Greenspan D S
Pan J
Weissman S M
Grumet F C
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00377967
Published
1986-00-00
Pages
90-9
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Grants
NIAID NIH HHS · AI06784 · United States
NIADDK NIH HHS · AM33233 · United States
NCI NIH HHS · CA30938 · United States
External Links
PubMed source DOI: 10.1007/BF00377967 View journal details
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