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PMID: 3004947 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The SV40 enhancer influences viral late transcription in vitro and in vivo but not on replicating templates.

The EMBO journal ·Vol. 4 ·No. 12 ·1985-12-01 ·Pages 3247-52

Shaw PE, Bohmann D, Sergeant A

Abstract

We have examined transcription from the SV40 late promoter in vitro and in vivo. In HeLa whole cell extracts, late transcription is efficient in the absence of T antigen but is impaired by enhancer specific point mutations. In vivo, when replication is prevented, transcription from the late promoter requires T antigen as well as a functional enhancer. However, enhancer sequences fail to potentiate late transcription from replicating templates although, under such conditions, enhancer binding factors do not become limiting. It appears that the SV40 late transcription unit is refractory to enhancer-mediated activation when it is located on a replicating template.

MeSH Terms
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Base Sequence DNA Replication DNA Restriction Enzymes Enhancer Elements, Genetic Genes, Regulator Genes, Viral HeLa Cells Humans Oncogene Proteins, Viral/genetics Promoter Regions, Genetic Protein Kinases/genetics Simian virus 40/genetics Templates, Genetic Transcription, Genetic
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Oncogene Proteins, Viral Protein Kinases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shaw P E
Bohmann D
Sergeant A
References (42)
42 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-01
Pages
3247-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554650
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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