Home LiteratureArticle Details
PMID: 2999772 Published · ppublish English Journal Article

High-affinity receptors for peptides of the bombesin family in Swiss 3T3 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 22 ·1985-11-00 ·Pages 7616-20

Zachary I, Rozengurt E

Abstract

Gastrin-releasing peptide (GRP) labeled with 125I at tyrosine-15 (125I-GRP) binds to intact quiescent Swiss 3T3 cells in a specific and saturable manner. Scatchard analysis indicates the presence of a single class of high-affinity binding sites of Kd = 0.5 X 10(-9) M and a value for the number of sites per cell of about 100,000. 125I-GRP binding was not inhibited by other mitogens for these cells, and cell lines that are mitogenically unresponsive to GRP do not exhibit specific GRP binding. Structure-activity relationships show a close parallel between the ability of a range of GRP-related peptides to both inhibit GRP binding and to stimulate mitogenesis. Further, GRP binding is selectively blocked in a competitive fashion by a novel bombesin antagonist, [D-Arg1, D-Pro2, D-Trp7,9, Leu11] substance P. In addition, this compound selectively inhibits GRP and bombesin-induced mitogenesis. These results demonstrate that the mitogenic response of Swiss 3T3 cells to peptides of the bombesin family is mediated by a class of receptors distinct from those of other mitogens for these cells.

MeSH Terms
Animals Bombesin/antagonists & inhibitors Carcinoma, Small Cell/drug therapy Cells, Cultured DNA/biosynthesis Gastrin-Releasing Peptide Iodine Radioisotopes Lung Neoplasms/drug therapy Mice Peptides/metabolism,pharmacology Platelet-Derived Growth Factor/pharmacology Receptors, Bombesin Receptors, Cell Surface/analysis
Chemicals
Iodine Radioisotopes Peptides Platelet-Derived Growth Factor Receptors, Bombesin Receptors, Cell Surface Gastrin-Releasing Peptide DNA Bombesin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zachary I
Rozengurt E
References (45)
45 references, click to expand
  1. Isolation and structure of bombesin and alytesin, 2 analogous active peptides from the skin of the European amphibians Bombina and Alytes.
    Experientia. 1971 Feb 15;27(2):166-7 PMID: 5544731
  2. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
    J Cell Biol. 1963 May;17:299-313 PMID: 13985244
  3. An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4555-9 PMID: 188046
  4. Bombesin, bombesin analogues, and related peptides: effects on thermoregulation.
    Biochemistry. 1978 May 2;17(9):1766-71 PMID: 656396
  5. Bombesin-like immunoreactivity in the lung.
    Nature. 1978 Jun 29;273(5665):769-70 PMID: 351419
  6. The platelet-derived growth factor.
    Cell. 1978 Jun;14(2):203-10 PMID: 352535
  7. Stimulation of DNA synthesis by tumour promoter and pure mitogenic factors.
    Nature. 1978 Dec 14;276(5689):723-6 PMID: 310518
  8. Interaction of bombesin and litorin with specific membrane receptors on pancreatic acinar cells.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6139-43 PMID: 216015
  9. Vasopressin stimulation of mouse 3T3 cell growth.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1284-7 PMID: 312501
  10. Bombesin-like peptides in mammals.
    Fed Proc. 1979 Aug;38(9):2315-9 PMID: 456618
  11. Chemical messengers: a view from the gut.
    Fed Proc. 1979 Aug;38(9):2341-3 PMID: 456621
  12. Bombesin suppresses feeding in rats.
    Nature. 1979 Nov 8;282(5735):208-10 PMID: 492336
  13. Characterization of a gastrin releasing peptide from porcine non-antral gastric tissue.
    Biochem Biophys Res Commun. 1979 Sep 12;90(1):227-33 PMID: 496973
  14. Bombesin-like peptides in rat brain: quantitation and biochemical characterization.
    Biochem Biophys Res Commun. 1979 Sep 12;90(1):7-14 PMID: 496992
  15. Hormonal regulation of peptide receptors and target cell responses.
    Nature. 1979 Jul 12;280(5718):109-16 PMID: 233264
  16. Phorbol esters and vasopressin stimulate DNA synthesis by a common mechanism.
    Nature. 1980 Oct 16;287(5783):607-12 PMID: 7432481
  17. Brain regulation of gastric secretion: influence of neuropeptides.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5515-9 PMID: 6159649
  18. Stimulation of DNA synthesis in quiescent cultured cells: exogenous agents, internal signals, and early events.
    Curr Top Cell Regul. 1980;17:59-88 PMID: 6254731
  19. Similarities between fibroblast-derived growth factor and platelet-derived growth factor.
    Exp Cell Res. 1981 Sep;135(1):221-7 PMID: 6456922
  20. Bombesin, somatostatin and neurotensin-like immunoreactivity in bronchial carcinoma.
    J Clin Endocrinol Metab. 1981 Dec;53(6):1310-2 PMID: 6117568
  21. High levels of intracellular bombesin characterize human small-cell lung carcinoma.
    Science. 1981 Dec 11;214(4526):1246-8 PMID: 6272398
  22. Inhibition of epidermal growth factor binding to mouse cultured cells by fibroblast-derived growth factor. Evidence for an indirect mechanism.
    J Biol Chem. 1982 Apr 10;257(7):3680-6 PMID: 6277944
  23. Human lung small-cell carcinoma contains bombesin.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2379-83 PMID: 6285381
  24. Regulatory peptides in the mammalian respiratory tract.
    Endocrinology. 1982 Oct;111(4):1248-54 PMID: 6180890
  25. Neuronal peptides in the intestine.
    Br Med Bull. 1982 Sep;38(3):247-52 PMID: 6756540
  26. Localization of regulatory peptides in the gut.
    Br Med Bull. 1982 Sep;38(3):303-7 PMID: 6129025
  27. A substance P antagonist inhibits vagally induced increase in vascular permeability and bronchial smooth muscle contraction in the guinea pig.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):1120-4 PMID: 6189120
  28. Bombesin stimulation of DNA synthesis and cell division in cultures of Swiss 3T3 cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):2936-40 PMID: 6344074
  29. Characterization of bombesin receptors in a rat pituitary cell line.
    J Biol Chem. 1983 Jun 25;258(12):7527-35 PMID: 6305945
  30. Neuromedin B: a novel bombesin-like peptide identified in porcine spinal cord.
    Biochem Biophys Res Commun. 1983 Jul 29;114(2):541-8 PMID: 6882442
  31. Platelet-derived growth factor elicits cyclic AMP accumulation in Swiss 3T3 cells: role of prostaglandin production.
    Cell. 1983 Aug;34(1):265-72 PMID: 6192934
  32. Gastrin-releasing peptide-related peptides in a human malignant lung carcinoid tumor.
    Cancer Res. 1983 Nov;43(11):5411-5 PMID: 6616473
  33. Phorbol esters, phospholipase C, and growth factors rapidly stimulate the phosphorylation of a Mr 80,000 protein in intact quiescent 3T3 cells.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7244-8 PMID: 6316349
  34. Molecular aspects of growth factor action: receptors and intracellular signals.
    J Pathol. 1983 Nov;141(3):309-31 PMID: 6363650
  35. Bombesin stimulates insulin secretion by a pancreatic islet cell line.
    Proc Natl Acad Sci U S A. 1984 Mar;81(6):1822-6 PMID: 6143320
  36. Gastrin-releasing peptide immunoreactivity in medullary thyroid carcinoma.
    Cancer. 1984 Jun 1;53(11):2472-7 PMID: 6370416
  37. A synthetic peptide that is a bombesin receptor antagonist.
    Nature. 1984 May 3-9;309(5963):61-3 PMID: 6201745
  38. Bombesin and the C-terminal tetradecapeptide of gastrin-releasing peptide are growth factors for normal human bronchial epithelial cells.
    Exp Cell Res. 1984 Jul;153(1):245-8 PMID: 6547391
  39. Growth factors, cell proliferation and cancer: an overview.
    Mol Biol Med. 1983 Jul;1(1):169-81 PMID: 6092824
  40. Neuromedin B is a major bombesin-like peptide in rat brain: regional distribution of neuromedin B and neuromedin C in rat brain, pituitary and spinal cord.
    Biochem Biophys Res Commun. 1984 Nov 14;124(3):925-32 PMID: 6548910
  41. Gastrin releasing peptide is a selective mitogen for small cell lung carcinoma in vitro.
    J Clin Invest. 1985 Jan;75(1):306-9 PMID: 2981251
  42. Autocrine growth factors and cancer.
    Nature. 1985 Feb 28-Mar 6;313(6005):745-7 PMID: 3883191
  43. Serum, like phorbol esters, rapidly activates protein kinase C in intact quiescent fibroblasts.
    EMBO J. 1985 Jan;4(1):71-6 PMID: 3160581
  44. Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer.
    Nature. 1985 Aug 29-Sep 4;316(6031):823-6 PMID: 2993906
  45. Membrane receptors for hormones and neurotransmitters.
    J Cell Biol. 1976 Aug;70(2 pt 1):261-86 PMID: 7569
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.22.7616
Published
1985-11-00
Pages
7616-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391384
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.22.7616 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2