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PMID: 2999701 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter selectivity of E. coli RNA polymerase: analysis of the promoter system of convergently-transcribed dnaQ-rnh genes.

Nucleic acids research ·Vol. 13 ·No. 21 ·1985-11-11 ·Pages 7647-61

Nomura T, Fujita N, Ishihama A

Abstract

Promoter properties were analyzed for the convergently-overlapped E. coli genes coding for the DNA polymerase III epsilon subunit (dnaQ) and the ribonuclease H (rnh). The rates of open complex formation for a single promoter of the rnh gene and two tandem promoters of the dnaQ gene were constant whether they are located on a single DNA fragment or separated into individual fragments. The relative expression levels of these three promoters, as measured using an in vitro mixed transcription system, varied differentially depending on the concentration of RNA polymerase. At low enzyme concentrations, the downstream promoter (P2) of the dnaQ gene was utilized preferentially, but the upstream promoter (P1) was utilized as well when the enzyme concentration was increased. This indicates different physiological roles between the two dnaQ promoters. The level of rnh transcription was as low as that of dnaQ-1 RNA synthesis but the rnh promoter was utilized as well as the dnaQ P2 promoter when it was separated from the dnaQ promoters. This implies a promoter interference between the convergently transcribed genes.

MeSH Terms
Base Sequence Cloning, Molecular DNA Polymerase III/genetics DNA Restriction Enzymes DNA-Directed DNA Polymerase/genetics DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology,genetics Genes Genes, Bacterial Kinetics Promoter Regions, Genetic Ribonucleases/genetics Transcription, Genetic
Chemicals
DNA-Directed RNA Polymerases DNA Polymerase III DNA-Directed DNA Polymerase Ribonucleases DNA Restriction Enzymes ribonuclease B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nomura T
Fujita N
Ishihama A
References (34)
34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.21.7647
Published
1985-11-11
Pages
7647-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322077
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.21.7647 PMC Full Text View journal details
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