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PMID: 2994065 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A highly polymorphic locus in human DNA revealed by cosmid-derived probes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 18 ·1985-09-00 ·Pages 6206-10

Litt M, White RL

Abstract

Human gene mapping would be greatly facilitated if marker loci with sufficient heterozygosity were generally available. As a source of such markers, we have used cosmids from a human genomic library. We have developed a rapid method for screening random cosmids to identify those that are homologous to genomic regions especially rich in restriction fragment length polymorphisms. This method allows whole cosmids to be used as probes against Southern transfers of genomic DNA; regions of cosmid probes homologous to repeated genomic sequences are rendered unable to hybridize with Southern transfers by prehybridization of the probes with a vast excess of nonradioactive genomic DNA. From one cosmid identified by this procedure, we have isolated three single-copy probes that collectively identify seven polymorphic loci. Of 56 unrelated individuals, 52 were heterozygous at one or more of these marker loci.

MeSH Terms
Chromosome Mapping/methods DNA/genetics DNA Restriction Enzymes DNA, Recombinant Genetic Linkage Humans Plasmids Polymorphism, Genetic
Chemicals
DNA, Recombinant DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Litt M
White R L
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.18.6206
Published
1985-09-00
Pages
6206-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391021
Subset
IM
Grants
NIGMS NIH HHS · GM 32500 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.18.6206 PMC Full Text View journal details
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