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PMID: 2991749 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells.

Molecular and cellular biology ·Vol. 5 ·No. 7 ·1985-00-00 ·Pages 1722-34

Merlino GT, Ishii S, Whang-Peng J, Knutsen T, Xu YH, Clark AJ, Stratton RH, Wilson RK, Ma DP, Roe BA, Hunts JH, Shimizu N, Pastan I

Abstract

A431 cells have an amplification of the epidermal growth factor (EGF) receptor gene, the cellular homolog of the v-erb B oncogene, and overproduce an aberrant 2.9-kilobase RNA that encodes a portion of the EGF receptor. A cDNA (pE15) for the aberrant RNA was cloned, sequenced, and used to analyze genomic DNA blots from A431 and normal cells. These data indicate that the aberrant RNA is created by a gene rearrangement within chromosome 7, resulting in a fusion of the 5' portion of the EGF receptor gene to an unidentified region of genomic DNA. The unidentified sequences are amplified to about the same degree (20- to 30-fold) as the EGF receptor sequences. In situ hybridization to chromosomes from normal cells and A431 cells show that both the EGF receptor gene and the unidentified DNA are localized to the p14-p12 region of chromosome 7. By using cDNA fragments to probe DNA blots from mouse-A431 somatic cell hybrids, the rearranged receptor gene was shown to be associated with translocation chromosome M4.

MeSH Terms
Amino Acid Sequence Base Sequence Carcinoma, Squamous Cell/genetics Cell Line Chromosomes, Human, 6-12 and X Cloning, Molecular DNA/genetics DNA, Neoplasm/genetics ErbB Receptors Gene Amplification Humans Karyotyping Nucleic Acid Conformation RNA, Messenger/genetics Receptors, Cell Surface/genetics Translocation, Genetic
Chemicals
DNA, Neoplasm RNA, Messenger Receptors, Cell Surface DNA ErbB Receptors
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Merlino G T
Ishii S
Whang-Peng J
Knutsen T
Xu Y H
Clark A J
Stratton R H
Wilson R K
Ma D P
Roe B A
Hunts John
Shimizu Nobuyoshi
Pastan Ira
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.7.1722-1734.1985
Published
1985-00-00
Pages
1722-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367291
Subset
IM
Grants
NIGMS NIH HHS · GM-24375 · United States
NIGMS NIH HHS · GM-30400 · United States
Databases
GENBANK
K01885, K02047, K03193, X00588, X00663
External Links
PubMed source DOI: 10.1128/mcb.5.7.1722-1734.1985 PMC Full Text View journal details
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