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PMID: 2988510 Published · ppublish English Journal Article

Breakdown of phosphatidylinositol 4,5-bisphosphate in a T-cell leukaemia line stimulated by phytohaemagglutinin is not dependent on Ca2+ mobilization.

The Biochemical journal ·Vol. 227 ·No. 3 ·1985-05-01 ·Pages 971-9

Sasaki T, Hasegawa-Sasaki H

Abstract

Addition of phytohaemagglutinin (PHA) to the [32P]Pi-prelabelled JURKAT cells, a human T-cell leukaemia line, resulted in a decrease of [32P]phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] to about 35% of the control value. The decrease was almost complete within 30s after the PHA addition. This decrease was followed by an increase in the 32P-labelling of phosphatidic acid (maximally 2.8-fold at 2 min). The stimulation of myo-[2-3H]inositol-prelabelled JURKAT cells by PHA induced an accumulation of [2-3H]inositol trisphosphate in the presence of 5 mM-LiCl. The result indicates hydrolysis of PtdIns (4,5)P2 by a phospholipase C. The PHA stimulation of JURKAT cells induced about 6-fold increase in the cytosolic free Ca2+ concentration, [Ca2+]i, which was reported by Quin-2, a fluorescent Ca2+ indicator. Studies with partially Ca2+-depleted JURKAT cells, with the Ca2+ ionophore A23187, and with 8-(diethylamino)-octyl-3,4,5-trimethoxybenzoate indicate that the breakdown of PtdIns(4,5)P2 is not mediated through changes of [Ca2+]i. These results therefore indicate that the PHA-induced breakdown of PtdIns(4,5)P2 in JURKAT cells is not dependent on the Ca2+ mobilization.

MeSH Terms
Agglutinins/pharmacology Aminoquinolines/pharmacology Animals Calcimycin/pharmacology Calcium/metabolism Cell Line Chromatography, Thin Layer Humans Leukemia, Experimental/metabolism Leukocytes Phosphatidic Acids/metabolism Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols/metabolism Proteins T-Lymphocytes/drug effects,metabolism Type C Phospholipases/metabolism
Chemicals
Agglutinins Aminoquinolines Phosphatidic Acids Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols Proteins leukoagglutinins, leukocytes Calcimycin Type C Phospholipases Quin2 Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sasaki T
Hasegawa-Sasaki H
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45 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2270971
Published
1985-05-01
Pages
971-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144929
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2270971 PMC Full Text View journal details
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