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PMID: 2988434 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Correlation of aminoglycoside resistance with the KmS and Vmax/Km ratios of enzymatic modification of aminoglycosides by 2''-O-nucleotidyltransferase.

Antimicrobial agents and chemotherapy ·Vol. 27 ·No. 4 ·1985-04-00 ·Pages 670-1

DeHertogh DA, Lerner SA

Abstract

A clinical isolate, Serratia marcescens 75, was found to be susceptible to netilmicin and yet had a high level of aminoglycoside 2''-O-nucleotidyltransferase activity for netilmicin. Kinetic studies of the partially purified enzyme revealed substrate inhibition for gentamicin and tobramycin at concentrations greater than 10(-2) mM, but this was not observed for netilmicin. The MICs of the aminoglycosides tested exhibited a good inverse correlation with the Km values for the enzyme and a direct correlation with the Vmax/Km ratios of the enzyme.

MeSH Terms
Aminoglycosides/pharmacology Anti-Bacterial Agents/pharmacology Drug Resistance, Microbial Gentamicins/metabolism Kinetics Netilmicin/pharmacology Nucleotidyltransferases/metabolism Serratia marcescens/enzymology Tobramycin/metabolism Ultrasonics
Chemicals
Aminoglycosides Anti-Bacterial Agents Gentamicins Netilmicin Nucleotidyltransferases gentamicin 2''-nucleotidyltransferase Tobramycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DeHertogh D A
Lerner S A
References (9)
9 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.27.4.670
Published
1985-04-00
Pages
670-1
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC180122
Subset
IM
Grants
NIAID NIH HHS · AI-07099 · United States
External Links
PubMed source DOI: 10.1128/AAC.27.4.670 PMC Full Text View journal details
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