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PMID: 2987871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A mitochondrial reading frame which may code for a maturase-like protein in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 13 ·No. 8 ·1985-04-25 ·Pages 3005-14

Séraphin B, Simon M, Faye G

Abstract

In S. cerevisiae, the large oxi3/oli2 mitochondrial transcript contains the products of the oxi3, aap1 and oli2 genes and an unassigned reading frame, RF3. In the work presented here, we have completed the nucleotide sequence of RF3. We have shown that RF3 is composed of four fairly large ORFs which overlap within GC rich sequences. Furthermore, a shift of +1 base was found between each pair of consecutive reading frames. We discuss how these frameshifts could be removed to produce a 500 aminoacid long protein containing the two well conserved P1 and P2 oligopeptide sequences featuring several mitochondrial intron reading frames, suggesting, thereby, a RNA-maturase-like activity for the putative RF3 protein. In addition, we suggest that the insertion of GC clusters in a gene could provide a novel way of regulating its expression.

MeSH Terms
Base Sequence Endoribonucleases Mitochondria/metabolism Nucleotidyltransferases/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Nucleotidyltransferases mRNA maturase Endoribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Séraphin B
Simon M
Faye G
References (34)
34 references, click to expand
  1. Leaky +1 and -1 frameshift mutations at the same site in a yeast mitochondrial gene.
    Nature. 1980 Nov 6;288(5786):60-3 PMID: 6253818
  2. Assembly of the mitochondrial membrane system. DNA sequence and organization of the cytochrome b gene in Saccharomyces cerevisiae D273-10B.
    J Biol Chem. 1980 Oct 25;255(20):9828-37 PMID: 6253454
  3. [Nucleotide sequence of the gene for the mitochondrial 15S ribosomal RNA of yeast].
    C R Seances Acad Sci D. 1980 Dec 8;291(12):933-6 PMID: 6261980
  4. Frameshift suppression.
    Cell. 1981 Jun;24(3):601-2 PMID: 6166384
  5. Assembly of the mitochondrial membrane system. Analysis of the nucleotide sequence and transcripts in the oxi1 region of yeast mitochondrial DNA.
    J Biol Chem. 1981 Dec 25;256(24):12780-7 PMID: 7031051
  6. Identification and suppression of secondary structures formed from deoxy-oligonucleotides during electrophoresis in denaturing polyacrylamide-gels.
    Nucleic Acids Res. 1981 Oct 10;9(19):4967-79 PMID: 7312623
  7. Lysis gene expression of RNA phage MS2 depends on a frameshift during translation of the overlapping coat protein gene.
    Nature. 1982 Jan 7;295(5844):35-41 PMID: 7066091
  8. Nature of an inserted sequence in the mitochondrial gene coding for the 15S ribosomal RNA of yeast.
    Nucleic Acids Res. 1982 Mar 11;10(5):1625-33 PMID: 6280154
  9. Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.
    Biochimie. 1982 Oct;64(10):867-81 PMID: 6817818
  10. Analysis of a yeast nuclear gene involved in the maturation of mitochondrial pre-messenger RNA of the cytochrome oxidase subunit I.
    Cell. 1983 Jan;32(1):77-87 PMID: 6297789
  11. Two intron sequences in yeast mitochondrial COX1 gene: homology among URF-containing introns and strain-dependent variation in flanking exons.
    Cell. 1983 Feb;32(2):379-89 PMID: 6297792
  12. Nucleotide sequence of Rous sarcoma virus.
    Cell. 1983 Mar;32(3):853-69 PMID: 6299578
  13. Expression of the "split gene" cob in yeast mtDNA. Nuclear mutations specifically block the excision of different introns from its primary transcript.
    J Biol Chem. 1983 Jul 10;258(13):7954-9 PMID: 6345534
  14. An mRNA maturase is encoded by the first intron of the mitochondrial gene for the subunit I of cytochrome oxidase in S. cerevisiae.
    Cell. 1983 Dec;35(3 Pt 2):733-42 PMID: 6317200
  15. Steps in processing of the mitochondrial cytochrome oxidase subunit I pre-mRNA affected by a nuclear mutation in yeast.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):8-12 PMID: 6320175
  16. Cytochrome b gene of Neurospora crassa mitochondria. Partial sequence and location of introns at sites different from those in Saccharomyces cerevisiae and Aspergillus nidulans.
    J Biol Chem. 1984 Jan 10;259(1):504-11 PMID: 6231283
  17. Processing of yeast mitochondrial messenger RNAs at a conserved dodecamer sequence.
    EMBO J. 1984 Apr;3(4):829-34 PMID: 6327291
  18. Expandable var1 gene of yeast mitochondrial DNA: in-frame insertions can explain the strain-specific protein size polymorphisms.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3148-52 PMID: 6328501
  19. Antibodies against a fused 'lacZ-yeast mitochondrial intron' gene product allow identification of the mRNA maturase encoded by the fourth intron of the yeast cob-box gene.
    EMBO J. 1984 Jul;3(7):1567-72 PMID: 6430696
  20. Three variant introns of the same general class in the mitochondrial gene for cytochrome oxidase subunit 1 in Aspergillus nidulans.
    EMBO J. 1984 Sep;3(9):2121-8 PMID: 6092056
  21. Organization and processing of the mitochondrial oxi3/oli2 multigenic transcript in yeast.
    Mol Gen Genet. 1984;196(2):266-74 PMID: 6387398
  22. The sequence of the gene for cytochrome c oxidase subunit I, a frameshift containing gene for cytochrome c oxidase subunit II and seven unassigned reading frames in Trypanosoma brucei mitochondrial maxi-circle DNA.
    Nucleic Acids Res. 1984 Oct 11;12(19):7327-44 PMID: 6093040
  23. A retrovirus-like strategy for expression of a fusion protein encoded by yeast transposon Ty1.
    Nature. 1985 Jan 17-23;313(5999):243-6 PMID: 2982101
  24. Nucleotide sequence and intron structure of the apocytochrome b gene of Neurospora crassa mitochondria.
    EMBO J. 1983;2(8):1235-42 PMID: 10872314
  25. Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.
    EMBO J. 1983;2(1):33-8 PMID: 11894905
  26. Assembly of the mitochondrial membrane system. XIX. Genetic characterization of mit- mutants with deficiencies in cytochrome oxidase and coenzyme qh2-cytochrome c reductase.
    Mol Gen Genet. 1976 Nov 24;149(1):43-50 PMID: 189177
  27. The mitochondrial genome of wild-type yeast cells. VI. Genome organization.
    J Mol Biol. 1977 Feb 15;110(1):53-74 PMID: 845947
  28. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  29. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  30. Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci.
    Cell. 1980 May;20(1):185-97 PMID: 6156002
  31. Assembly of the mitochondrial membrane system: sequence analysis of a yeast mitochondrial ATPase gene containing the oli-2 and oli-4 loci.
    Cell. 1980 Jun;20(2):507-17 PMID: 6446405
  32. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.
    Gene. 1980 May;9(3-4):287-305 PMID: 6248430
  33. Assembly of the mitochondrial membrane system. Sequences of yeast mitochondrial tRNA genes.
    J Biol Chem. 1980 Oct 10;255(19):9075-81 PMID: 6251074
  34. Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrme oxidase.
    J Biol Chem. 1980 Dec 25;255(24):11927-41 PMID: 6254986
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.8.3005
Published
1985-04-25
Pages
3005-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341210
Subset
IM
Databases
GENBANK
X02421
External Links
PubMed source DOI: 10.1093/nar/13.8.3005 PMC Full Text View journal details
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