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PMID: 2987811 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a mouse interferon gene locus I. Isolation of a cluster of four alpha interferon genes.

Nucleic acids research ·Vol. 13 ·No. 3 ·1985-02-11 ·Pages 805-23

Kelley KA, Pitha PM

Abstract

A BALB/c mouse genomic library was screened with a murine interferon alpha 2 (MuIFN-alpha 2) cDNA coding region fragment. Eight clones were isolated which contain different mouse chromosomal segments related to the MuIFN-alpha 2 probe and a 28 kilobase (kb) region of mouse genomic DNA containing four different MuIFN-alpha genes (alpha 1, alpha 4, alpha 5 and alpha 6) was identified and characterized; an intergenic 1000 nucleotide long conserved sequence was found to be associated with three of these four alpha genes, indicating that this alpha-IFN gene cluster evolved through tandem duplications. Sequence analysis revealed the absence of a polyadenylation site in the 3' untranslated region of MuIFN-alpha 1, and showed that one of the genes (alpha 4) contains an internal deletion of 5 amino acids in the coding region.

MeSH Terms
Animals Base Sequence Chromosome Mapping DNA Restriction Enzymes/metabolism Interferon Type I/genetics Mice Mice, Inbred BALB C Repetitive Sequences, Nucleic Acid
Chemicals
Interferon Type I DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kelley K A
Pitha P M
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.3.805
Published
1985-02-11
Pages
805-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341036
Subset
IM
Grants
NIAID NIH HHS · 5OI-AI-19737-02 · United States
Databases
GENBANK
M23828, M23829, M23830, M23831, M23925, M23926
External Links
PubMed source DOI: 10.1093/nar/13.3.805 PMC Full Text View journal details
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