Primary structure and gene organization of human hepatitis A virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 9 ·1985-05-00 ·Pages 2627-31

Najarian R, Caput D, Gee W, Potter SJ, Renard A, Merryweather J, Van Nest G, Dina D

Abstract

The RNA genome of human hepatitis A virus (HAV) was molecularly cloned. Recombinant DNA clones representing the entire HAV RNA were used to determine the primary structure of the viral genome. The length of the viral genome is 7478 nucleotides. An open reading frame starting at nucleotide 734 and terminating at nucleotide 7415 encodes a polyprotein of Mr 251,940. Comparison of the HAV nucleotide sequence with that of other picornaviruses has failed to reveal detectable areas of homology. However, a computer analysis of the putative amino acid sequence of HAV and poliovirus demonstrated the existence of short areas of homology in virion protein 3 (VP3) and throughout the carboxyl-terminal portion of the polyproteins. In addition, extensive protein structural homologies with poliovirus were detected.

MeSH Terms

Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Genes, Viral Hepatovirus/genetics Poliovirus/genetics RNA, Viral/genetics Species Specificity Viral Proteins/genetics Viral Structural Proteins

Chemicals

RNA, Viral Viral Proteins Viral Structural Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Najarian R

Caput D

Gee W

Potter S J

Renard A

Merryweather J

Van Nest G

Dina D

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.9.2627

Published

1985-05-00

Pages

2627-31

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397617

Subset

Databases

GENBANK

K02990

External Links

PubMed source DOI: 10.1073/pnas.82.9.2627 PMC Full Text View journal details

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Primary structure and gene organization of human hepatitis A virus.

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