Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells.
Synchronized CV-1 cells were transfected with SV40 (simian virus 40) DNA-calcium phosphate co-precipitates. In the presence of carrier DNA, the transfection efficiency of SV40 DNA was decreased 5-fold in S-phase cells and was increased 4-fold in preparations of mitotically enriched cells as compared with asynchronous controls. No difference was observed when carrier DNA was omitted, when cells had progressed through S-phase and into G2-phase, or when the infectivity of cells to intact SV40 virus was tested. These results highlight the importance of cell-cycle-dependent factors on DNA-mediated gene transfer.
18 references, click to expand
-
Liposome-mediated delivery of deoxyribonucleic acid to cells: enhanced efficiency of delivery related to lipid composition and incubation conditions.Biochemistry. 1981 Nov 24;20(24):6978-87 PMID: 6274382
-
Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review.Gene. 1981 Jun-Jul;14(1-2):1-10 PMID: 7262562
-
High efficiency polyoma DNA transfection of chloroquine treated cells.Nucleic Acids Res. 1983 Mar 11;11(5):1295-308 PMID: 6298741
-
Nuclear matrix: a cell-cycle-dependent site of increased intranuclear protein phosphorylation.Eur J Biochem. 1983 Jun 1;133(1):23-9 PMID: 6852029
-
Phosphorylation of nuclear proteins.Philos Trans R Soc Lond B Biol Sci. 1983 Jul 5;302(1108):143-50 PMID: 6137002
-
DNA-mediated gene transfer is more efficient during S-phase of the cell cycle.Biochem Biophys Res Commun. 1984 Jan 13;118(1):310-6 PMID: 6320829
-
The uptake and stability of simian-virus-40 DNA after calcium phosphate transfection of CV-1 cells.Biochem J. 1984 Mar 1;218(2):475-82 PMID: 6324757
-
Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells.Exp Cell Res. 1963 Apr;30:344-62 PMID: 13980637
-
VARIATIONS IN X-RAY RESPONSE DURING THE DIVISION CYCLE OF PARTIALLY SYNCHRONIZED CHINESE HAMSTER CELLS IN CULTURE.Nature. 1963 Sep 21;199:1158-60 PMID: 14072031
-
MITOTICALLY SYNCHRONIZED MAMMALIAN CELLS: A SIMPLE METHOD FOR OBTAINING LARGE POPULATIONS.Science. 1964 May 29;144(3622):1152-3 PMID: 14148440
-
Inhibition of DNA synthesis in HeLa cells by hydroxyurea.Cancer Res. 1967 Jan;27(1):124-9 PMID: 6020353
-
Commentary. Lysosomotropic agents.Biochem Pharmacol. 1974 Sep 15;23(18):2495-531 PMID: 4606365
-
Regulatory mechanism of simian virus 40 gene expression in permissive and in nonpermissive cells.J Virol. 1976 Mar;17(3):854-8 PMID: 176449
-
Nuclear localisation of an oocyte component required for the stability of injected DNA.Nature. 1977 Jul 14;268(5616):150-2 PMID: 201847
-
Characterization of a CV-1 cell cycle. III. Biophysical parameters.Exp Cell Res. 1979 Aug;122(1):1-8 PMID: 113229
-
Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA.Proc Natl Acad Sci U S A. 1980 Jan;77(1):433-6 PMID: 6244558
-
Genetic and physical linkage of exogenous sequences in transformed cells.Cell. 1980 Nov;22(1 Pt 1):309-17 PMID: 6253083
-
Regulation of SV40 gene expression.Adv Cancer Res. 1981;35:111-49 PMID: 6280457
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
