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PMID: 2974920 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.

Molecular and cellular biology ·Vol. 8 ·No. 11 ·1988-11-00 ·Pages 4787-91

Hamm J, van Santen VL, Spritz RA, Mattaj IW

Abstract

The binding of the U1 small nuclear ribonucleoprotein (snRNP)-specific proteins C, A, and 70K to U1 small nuclear RNA (snRNA) was analyzed. Assembly of U1 snRNAs from bean and soybean and a set of mutant Xenopus U1 snRNAs into U1 snRNPs in Xenopus egg extracts was studied. The ability to bind proteins was analyzed by immunoprecipitation with monospecific antibodies and by a protein-sequestering assay. The only sequence essential for binding of the U1-specific proteins was the conserved loop sequence in the 5' hairpin of U1. Further analysis suggested that protein C binds directly to the loop and that the assembly of proteins A and 70K into the RNP requires mainly protein-protein interactions. Protein C apparently recognizes a specific RNA sequence rather than a secondary structural element in the RNA.

MeSH Terms

Animals Base Sequence Binding Sites Molecular Sequence Data Mutation Nucleic Acid Conformation Plants Protein Binding RNA, Small Nuclear/metabolism Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear Species Specificity Xenopus laevis

Chemicals

RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hamm J

European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

van Santen V L

Spritz R A

Mattaj I W

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23 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.8.11.4787-4791.1988

Published

1988-11-00

Pages

4787-91

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC365571

Subset

External Links

PubMed source DOI: 10.1128/mcb.8.11.4787-4791.1988 PMC Full Text View journal details

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Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.

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