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PMID: 2973607 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 23 ·1988-12-00 ·Pages 9138-42

Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J

Abstract

Chromosomes can be specifically stained in metaphase spreads and interphase nuclei by in situ hybridization with entire chromosome-specific DNA libraries. Unlabeled human genomic DNA is used to inhibit the hybridization of sequences in the library that bind to multiple chromosomes. The target chromosome can be made at least 20 times brighter per unit length than the others. Trisomy 21 and translocations involving chromosome 4 can be detected in metaphase spreads and interphase nuclei by using this technique.

MeSH Terms
Cells, Cultured Chromosomes, Human, Pair 4 DNA/genetics Down Syndrome/genetics Fluorescent Antibody Technique Humans Lymphocytes/cytology Nucleic Acid Hybridization Translocation, Genetic
Chemicals
DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pinkel D
Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA 94550.
Landegent J
Collins C
Fuscoe J
Segraves R
Lucas J
Gray J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.23.9138
Published
1988-12-00
Pages
9138-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282679
Subset
IM
Grants
NCI NIH HHS · CA 45919 · United States
NICHD NIH HHS · HD 17655 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.23.9138 PMC Full Text View journal details
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