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PMID: 2968050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The immunopathology of acute experimental allergic encephalomyelitis. V. A light microscopic and ultrastructural immunohistochemical analysis of fibronectin and fibrinogen.

The American journal of pathology ·Vol. 131 ·No. 3 ·1988-06-00 ·Pages 547-58

Sobel RA, Schneeberger EE, Colvin RB

Abstract

Cellular expression and extracellular deposition of fibronectin and fibrin/fibrinogen in experimental allergic encephalomyelitis (EAE) in guinea pigs (GP) were studied by light and electron microscopic immunohistochemistry of the central nervous system (CNS). Normal GPs had few fibronectin+ and fibrin/fibrinogen+ CNS vessels. Positively stained vessels were more numberous in sensitized GPs prior to and after onset of EAE, and there were extracellular deposits of these molecules in inflammatory foci. Staining was greater in EAE-susceptible strain 13 GPs than in sensitized EAE-resistant strain 2 GP. On ultrastructural analysis both molecules were localized on endothelial cell luminal surfaces and on intravascular mononuclear cells. Fibronectin and the macrophage fibronectin receptor (A6F10) were found on mononuclear cells in parenchyma. Endothelial cell luminal surface fibronectin and fibrin/fibrinogen may mark and/or contribute to early immune events in EAE, including mononuclear cell margination and emigration in inflammatory sites. Genetic factors, cytokines, and components of coagulation may regulate these interactions in vivo.

MeSH Terms
Acute Disease Animals Encephalomyelitis, Autoimmune, Experimental/immunology,metabolism,pathology Fibrin/metabolism Fibrinogen/metabolism Fibronectins/metabolism Immunohistochemistry Macrophages/metabolism Microscopy, Electron Receptors, Fibronectin Receptors, Immunologic/metabolism
Chemicals
Fibronectins Receptors, Fibronectin Receptors, Immunologic Fibrin Fibrinogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sobel R A
Immunopathology Unit, Massachusetts General Hospital, Boston 02114.
Schneeberger E E
Colvin R B
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1988-06-00
Pages
547-58
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1880690
Subset
IM
Grants
NCI NIH HHS · CA-20822 · United States
NHLBI NIH HHS · HL-31577 · United States
NINDS NIH HHS · NS-00858 · United States
External Links
PubMed source PMC Full Text View journal details
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