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PMID: 2963539 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of DNA markers in the direction of the Huntington disease gene from the G8 locus.

American journal of human genetics ·Vol. 42 ·No. 2 ·1988-02-00 ·Pages 335-44

Smith B, Skarecky D, Bengtsson U, Magenis RE, Carpenter N, Wasmuth JJ

Abstract

To facilitate identification of additional DNA markers near and on opposite sides of the Huntington disease (HD) gene, we developed a panel of somatic-cell hybrids that allows accurate subregional mapping of DNA fragments in the distal portion of 4p. By means of the hybrid-cell mapping panel and a library of DNA fragments enriched for sequences from the terminal one-third of the short arm of chromosome 4, 105 DNA fragments were mapped to six different physical regions within 4p15-4pter. Four polymorphic DNA fragments of particular interest were identified, at least three of which are distal to the HD-linked D4S10 (G8) locus, a region of 4p previously devoid of DNA markers. Since the HD gene has also recently been shown to be distal to G8, these newly identified DNA markers are in the direction of the HD gene from G8, and one or more of them may be on the opposite side of HD from G8.

MeSH Terms
Animals Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 4 DNA/genetics Genetic Linkage Genetic Markers Humans Huntington Disease/genetics Hybrid Cells Nucleic Acid Hybridization
Chemicals
Genetic Markers DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Smith B
Department of Biological Chemistry, California College of Medicine, University of California, Irvine 92717.
Skarecky D
Bengtsson U
Magenis R E
Carpenter N
Wasmuth J J
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19 references, click to expand
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1988-02-00
Pages
335-44
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1715250
Subset
IM
Grants
NIGMS NIH HHS · GM25339 · United States
External Links
PubMed source PMC Full Text View journal details
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