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PMID: 2952538 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transfusions enriched for W3/25+ helper/inducer T lymphocytes prevent spontaneous diabetes in the BB/W rat.

Diabetologia ·Vol. 30 ·No. 1 ·1987-01-00 ·Pages 22-6

Mordes JP, Gallina DL, Handler ES, Greiner DL, Nakamura N, Pelletier A, Rossini AA

Abstract

Transfusions of spleen cells are known to prevent spontaneous autoimmune diabetes in susceptible BB/W rats, while T cell-depleted transfusions are ineffective. To characterize further the protective cell(s), we transfused young diabetes prone rats with splenocytes from diabetes resistant BB/W rats that were treated in vitro to enrich them in either OX8+ (suppressor/cytotoxic) T cells or W3/25+ (helper/inducer) T cells. Diabetes subsequently occurred in 19 of 29 (66%) recipients of OX8-enriched, W3/25-depleted cells and 20 of 37 (54%) controls, but in only 7 of 30 (23%) recipients of W3/25-enriched, OX8-depleted cells (p less than 0.005). Transfusion of spleen cells from diabetes resistant donor rats pretreated in vivo to deplete OX8+ cells also prevented diabetes in susceptible BB/W recipients. We conclude that transfusions of W3/25+ helper/inducer splenic T lymphocytes obtained from diabetes resistant animals prevent spontaneous diabetes in the BB/W rat.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Diabetes Mellitus/immunology,physiopathology,prevention & control Female Male Rats Rats, Inbred BB Spleen/cytology T-Lymphocytes, Cytotoxic/physiology T-Lymphocytes, Helper-Inducer/physiology T-Lymphocytes, Regulatory/physiology
Chemicals
Antibodies, Monoclonal
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mordes J P
Gallina D L
Handler E S
Greiner D L
Nakamura N
Pelletier A
Rossini A A
References (19)
19 references, click to expand
  1. Lymphocyte transfusions prevent diabetes in the Bio-Breeding/Worcester rat.
    J Clin Invest. 1984 Jul;74(1):39-46 PMID: 6610693
  2. Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies.
    Immunol Rev. 1983;74:57-82 PMID: 6226588
  3. Lymphopenia and abnormal lymphocyte subsets in the "BB" rat: relationship to the diabetic syndrome.
    Endocrinology. 1982 May;110(5):1825-7 PMID: 6978807
  4. Spleen cell transfusion in the Bio-Breeding/Worcester rat. Prevention of diabetes, major histocompatibility complex restriction, and long-term persistence of transfused cells.
    J Clin Invest. 1986 Apr;77(4):1399-401 PMID: 3958191
  5. Transfusions of whole blood prevent spontaneous diabetes mellitus in the BB/W rat.
    Science. 1983 Feb 25;219(4587):975-7 PMID: 6823559
  6. The BB diabetic rat. Profound T-cell lymphocytopenia.
    Diabetes. 1981 Oct;30(10):887-9 PMID: 6974111
  7. Spontaneous autoimmune diabetes mellitus in the BioBreeding/Worcester rat.
    Surv Synth Pathol Res. 1984;3(2):131-8 PMID: 6390618
  8. The diabetic syndrome of the 'BB' Wistar rat: possible relevance to type 1 (insulin-dependent) diabetes in man.
    Diabetologia. 1982 Apr;22(4):225-32 PMID: 7047269
  9. Prevention of diabetes in BioBreeding/Worcester rats with monoclonal antibodies that recognize T lymphocytes or natural killer cells.
    J Exp Med. 1986 Oct 1;164(4):1145-59 PMID: 3531381
  10. Administration of silica prevents diabetes in BB-rats.
    Diabetes. 1985 Feb;34(2):197-9 PMID: 2981751
  11. Expression of T cell differentiation antigens and Ia on rat cytotoxic T lymphocytes.
    J Immunol. 1982 Feb;128(2):580-4 PMID: 6976382
  12. Time course of the lymphopenia in BB rats. Relation to the onset of diabetes.
    Diabetes. 1985 Oct;34(10):955-9 PMID: 2931313
  13. MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses.
    Eur J Immunol. 1984 Mar;14(3):260-7 PMID: 6231186
  14. Identification of profound peripheral T lymphocyte immunodeficiencies in the spontaneously diabetic BB rat.
    J Immunol. 1983 Apr;130(4):1723-31 PMID: 6220066
  15. Immunology of insulin-dependent diabetes mellitus.
    Annu Rev Immunol. 1985;3:289-320 PMID: 3904771
  16. Absence of the RT-6 T cell subset in diabetes-prone BB/W rats.
    J Immunol. 1986 Jan;136(1):148-51 PMID: 3079605
  17. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
    J Exp Med. 1985 Jul 1;162(1):117-27 PMID: 3159821
  18. T lymphocytes of young and aged rats. I. Distribution, density, and capping of T antigens.
    J Immunol. 1981 Jul;127(1):149-53 PMID: 6972406
  19. Two subsets of rat T lymphocytes defined with monoclonal antibodies.
    Eur J Immunol. 1980 Aug;10(8):609-15 PMID: 6967416
Article Info
Journal
Diabetologia
Abbr.
Diabetologia
ISSN
0012-186X
DOI
10.1007/BF01788902
Published
1987-01-00
Pages
22-6
Language
English
Region
Germany
NLM ID
0006777
Subset
IM
Grants
NIADDK NIH HHS · AM 25306 · United States
NIADDK NIH HHS · AM 4-2254 · United States
External Links
PubMed source DOI: 10.1007/BF01788902 View journal details
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