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PMID: 3531381 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Prevention of diabetes in BioBreeding/Worcester rats with monoclonal antibodies that recognize T lymphocytes or natural killer cells.

The Journal of experimental medicine ·Vol. 164 ·No. 4 ·1986-10-01 ·Pages 1145-59

Like AA, Biron CA, Weringer EJ, Byman K, Sroczynski E, Guberski DL

Abstract

Diabetes-prone BioBreeding/Worcester (BB/Wor) rats received thrice weekly injections of mAb against antigens expressed on the surface of all T cells (OX19), cytotoxic/suppressor, and NK cells (OX8), helper/inducer cells (W3/25, OX35, OX38), and Ia+ cells (OX6, 3JP, OX17). Treatment with OX8 or OX19 achieved stable reductions of splenic and peripheral blood NK cells and helper/inducer T lymphocytes, respectively, and protected against diabetes. OX19 injections also prevented lymphocytic insulitis, thyroiditis, and the synthesis of autoantibodies to thyroid colloid and smooth muscle antigens. OX8 injections reduced splenic NK-mediated YAC-1 cell lysis, but did not prevent insulitis, thyroiditis, or autoantibody synthesis. Injections of mAb specific for antigens on the surface of helper/inducer cells, and for cells expressing IaE antigens provided marginal protection against diabetes without reductions of phenotypic subsets. These findings suggest that pancreatic beta cell destruction in the spontaneously diabetic BB/Wor rat is mediated by the combined action of NK and helper/inducer cells.

MeSH Terms
Animals Antibodies, Monoclonal/immunology,therapeutic use Antigens, Surface/immunology Autoantibodies/biosynthesis Diabetes Mellitus, Experimental/immunology,prevention & control Immunoglobulin G/classification Islets of Langerhans/pathology Killer Cells, Natural/immunology Rats T-Lymphocytes/classification,immunology
Chemicals
Antibodies, Monoclonal Antigens, Surface Autoantibodies Immunoglobulin G
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Like A A
Biron C A
Weringer E J
Byman K
Sroczynski E
Guberski D L
References (35)
35 references, click to expand
  1. Blastogenesis of natural killer cells during viral infection in vivo.
    J Immunol. 1982 Dec;129(6):2788-95 PMID: 7142707
  2. Spontaneous diabetes mellitus syndrome in the rat. I. Association with the major histocompatibility complex.
    J Exp Med. 1981 Oct 1;154(4):1237-42 PMID: 7026724
  3. Cyclosporin prevents diabetes in BB Wistar rats.
    Lancet. 1983 Jan 1;1(8314-5):10-2 PMID: 6129365
  4. Islet cell surface antibodies and lymphocyte antibodies in the spontaneously diabetic BB Wistar rat.
    Diabetes. 1982 Mar;31(3):278-81 PMID: 6759244
  5. Transfusions of whole blood prevent spontaneous diabetes mellitus in the BB/W rat.
    Science. 1983 Feb 25;219(4587):975-7 PMID: 6823559
  6. Autoantibodies in the BB/W rat.
    Diabetes. 1982 Sep;31(9):816-20 PMID: 6761216
  7. Lymphocytic thyroiditis and diabetes in the BB/W rat. A new model of autoimmune endocrinopathy.
    Diabetes. 1981 Dec;30(12):1058-61 PMID: 6895508
  8. Dynamic time course studies of the spontaneously diabetic BB Wistar rat. III. Light-microscopic and ultrastructural observations of pancreatic islets of Langerhans.
    Am J Pathol. 1982 Feb;106(2):237-49 PMID: 7039331
  9. Neonatal thymectomy prevents spontaneous diabetes mellitus in the BB/W rat.
    Science. 1982 May 7;216(4546):644-6 PMID: 7041259
  10. Lymphopenia and abnormal lymphocyte subsets in the "BB" rat: relationship to the diabetic syndrome.
    Endocrinology. 1982 May;110(5):1825-7 PMID: 6978807
  11. Spontaneous diabetes mellitus in the BB/W rat. Effects of glucocorticoids, cyclosporin-A, and antiserum to rat lymphocytes.
    Diabetes. 1983 Apr;32(4):326-30 PMID: 6832488
  12. Analytical and functional studies on the T cells of untreated and immunologically tolerant diabetes-prone BB rats.
    J Immunol. 1983 May;130(5):2168-72 PMID: 6220083
  13. Passive transfer of diabetes in the BB/W rat.
    Science. 1983 May 13;220(4598):727-8 PMID: 6836309
  14. Genetic analysis of the BB/W diabetic rat.
    Can J Genet Cytol. 1983 Feb;25(1):7-15 PMID: 6839196
  15. The onset and progression of pancreatic insulitis in the overt, spontaneously diabetic, young adult BB rat studied by pancreatic biopsy.
    Diabetes. 1984 Jan;33(1):33-6 PMID: 6360766
  16. Separation and isolation of rat natural killer (NK) cells from T cells with monoclonal antibodies.
    J Immunol. 1984 May;132(5):2183-4 PMID: 6609190
  17. Total lymphoid irradiation prevents diabetes mellitus in the Bio-Breeding/Worcester (BB/W) rat.
    Diabetes. 1984 Jun;33(6):543-7 PMID: 6373460
  18. Experimental allergic encephalomyelitis: successful treatment in vivo with a monoclonal antibody that recognizes T helper cells.
    J Immunol. 1984 Oct;133(4):1938-42 PMID: 6236260
  19. Prevention of diabetes mellitus in the BB/W rat with Cyclosporin-A.
    Am J Pathol. 1984 Oct;117(1):92-7 PMID: 6385729
  20. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo.
    Nature. 1984 Dec 6-12;312(5994):548-51 PMID: 6150440
  21. Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker.
    Science. 1985 Jan 25;227(4685):415-7 PMID: 3155574
  22. Adoptive transfer of autoimmune diabetes mellitus in biobreeding/Worcester (BB/W) inbred and hybrid rats.
    J Immunol. 1985 Mar;134(3):1583-7 PMID: 3968427
  23. Successful treatment of autoimmunity in NZB/NZW F1 mice with monoclonal antibody to L3T4.
    J Exp Med. 1985 Feb 1;161(2):378-91 PMID: 3919141
  24. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
    J Exp Med. 1985 Jul 1;162(1):117-27 PMID: 3159821
  25. In vivo prevention of thyroid and pancreatic autoimmunity in the BB rat by antibody to class II major histocompatibility complex gene products.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6627-31 PMID: 3901005
  26. Deficiency of phenotypic cytotoxic-suppressor T lymphocytes in the BB/W rat.
    J Immunol. 1986 Feb 1;136(3):856-9 PMID: 2934480
  27. MRC OX-52: a rat T-cell antigen.
    Immunology. 1986 Apr;57(4):527-31 PMID: 2937721
  28. The spontaneously diabetic Wistar rat. Metabolic and morphologic studies.
    Diabetes. 1977 Feb;26(2):100-12 PMID: 320072
  29. Spontaneous diabetes mellitus: reversal and prevention in the BB/W rat with antiserum to rat lymphocytes.
    Science. 1979 Dec 21;206(4425):1421-3 PMID: 388619
  30. Two subsets of rat T lymphocytes defined with monoclonal antibodies.
    Eur J Immunol. 1980 Aug;10(8):609-15 PMID: 6967416
  31. Natural killer (NK) cell activity in the rat. I. Isolation and characterization of the effector cells.
    J Immunol. 1981 Jul;127(1):282-7 PMID: 7240744
  32. Spontaneous diabetes in rats: destruction of islets is prevented by immunological tolerance.
    Science. 1981 Sep 18;213(4514):1390-2 PMID: 6791286
  33. Prevention of diabetes in rats by bone marrow transplantation.
    Ann Surg. 1981 Sep;194(3):328-38 PMID: 6791599
  34. The BB diabetic rat. Profound T-cell lymphocytopenia.
    Diabetes. 1981 Oct;30(10):887-9 PMID: 6974111
  35. The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice.
    J Exp Med. 1983 Jan 1;157(1):202-18 PMID: 6600267
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.164.4.1145
Published
1986-10-01
Pages
1145-59
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188403
Subset
IM
Grants
NIADDK NIH HHS · AM 19155 · United States
NIADDK NIH HHS · AM4-2254 · United States
NCI NIH HHS · CA41268 · United States
External Links
PubMed source DOI: 10.1084/jem.164.4.1145 PMC Full Text View journal details
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