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PMID: 2942940 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A partial genomic DNA clone for the alpha subunit of the mouse complement receptor type 3 and cellular adhesion molecule Mac-1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5644-8

Sastre L, Roman JM, Teplow DB, Dreyer WJ, Gee CE, Larson RS, Roberts TM, Springer TA

Abstract

A genomic clone coding for the alpha subunit of the mouse complement receptor type 3 and the cellular adhesion molecule Mac-1 has been isolated directly from a genomic library using synthetic oligonucleotide probes based on the amino-terminal amino acid sequence of the protein. The identity of the clone has been established by DNA sequencing and in vitro translation of hybrid-selected mRNA. The gene is present in a single copy in the murine genome. The region containing the amino-terminal exon has been sequenced. RNA gel blotting shows that the Mac-1 alpha-subunit mRNA is 6 kilobases in length. Mac-1 alpha-subunit mRNA is present in macrophages but not T lymphoma or L cells. During gamma interferon-stimulated maturation of the mouse premyelocytic cell line M1, Mac-1 alpha-subunit mRNA is induced. This corresponds with the tissue distribution of the Mac-1 alpha subunit, showing expression is regulated at least partially at the message level.

MeSH Terms
Animals Antigens, Surface/genetics Cell Adhesion Cell Differentiation/drug effects Cell Line Cloning, Molecular Gene Expression Regulation/drug effects Genes Interferon-gamma/pharmacology Leukocytes/physiology Macromolecular Substances Macrophage-1 Antigen Mice Molecular Weight Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Receptors, Complement/genetics Receptors, Complement 3b Transcription, Genetic
Chemicals
Antigens, Surface Macromolecular Substances Macrophage-1 Antigen RNA, Messenger Receptors, Complement Receptors, Complement 3b Interferon-gamma
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sastre L
Roman J M
Teplow D B
Dreyer W J
Gee C E
Larson R S
Roberts T M
Springer T A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5644
Published
1986-08-00
Pages
5644-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386345
Subset
IM
Grants
NIAID NIH HHS · AI20316 · United States
NIAID NIH HHS · AI22304 · United States
NCI NIH HHS · CA31799 · United States
Databases
GENBANK
M14293
External Links
PubMed source DOI: 10.1073/pnas.83.15.5644 PMC Full Text View journal details
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