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PMID: 2939450 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Methyl-directed repair of frameshift mutations in heteroduplex DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 10 ·1986-05-00 ·Pages 3395-7

Dohet C, Wagner R, Radman M

Abstract

DNA heteroduplexes with single unpaired bases of the four different kinds were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system can recognize and repair heteroduplexes with single unpaired bases--i.e., frameshift/wild-type heteroduplexes. The repair of a particular strand of the heteroduplex is inhibited by full methylation of the adenines in the GATC sequences of that strand. Thus, it appears that the E. coli mismatch repair system can act on newly synthesized DNA strands to remove replication errors involving the insertion or deletion of a single base.

MeSH Terms

Bacteriophage lambda/genetics DNA Repair DNA Replication DNA, Bacterial/genetics Escherichia coli/genetics Methylation Mutation

Chemicals

DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dohet C

Wagner R

Radman M

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.10.3395

Published

1986-05-00

Pages

3395-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC323520

Subset

External Links

PubMed source DOI: 10.1073/pnas.83.10.3395 PMC Full Text View journal details

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Methyl-directed repair of frameshift mutations in heteroduplex DNA.

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