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PMID: 2930500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification of the neutral proteoglycan-degrading metalloproteinase from human articular cartilage tissue and its identification as stromelysin matrix metalloproteinase-3.

The Biochemical journal ·Vol. 258 ·No. 1 ·1989-02-15 ·Pages 115-9

Gunja-Smith Z, Nagase H, Woessner JF

Abstract

The 'neutral' proteoglycan-degrading metalloproteinase of human articular cartilage was purified 3,500-fold by use of an anti-(matrix metalloproteinase-3) immunoglobulin G affinity column. Molecular masses of the latent and multiple active forms and specificity of action on casein, transferrin, gelatin and fibronectin were identical with those of authentic stromelysin (matrix metalloproteinase-3) from cultured human rheumatoid synovial fibroblasts. The optimum pH of this proteinase on proteoglycan monomer was pH 5.5, and on Azocoll, 6.2; digestion of fibronectin and gelatin was more extensive at pH 5.5 than at 7.5.

MeSH Terms

Cartilage, Articular/analysis Humans Hydrogen-Ion Concentration Matrix Metalloproteinase 3 Metalloendopeptidases/isolation & purification,metabolism Proteoglycans/metabolism Substrate Specificity

Chemicals

Proteoglycans Metalloendopeptidases Matrix Metalloproteinase 3

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gunja-Smith Z

Department of Medicine, University of Miami School of Medicine, FL 33101.

Nagase H

Woessner J F

References (22)

22 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2580115

Published

1989-02-15

Pages

115-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1138331

Subset

Grants

NIAMS NIH HHS · AR-16940 · United States

NIAMS NIH HHS · AR-39189 · United States

External Links

PubMed source DOI: 10.1042/bj2580115 PMC Full Text View journal details

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Purification of the neutral proteoglycan-degrading metalloproteinase from human articular cartilage tissue and its identification as stromelysin matrix metalloproteinase-3.

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