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PMID: 6286237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Preparation of a monospecific antibody to purified rabbit synovial fibroblast collagenase.

Collagen and related research ·Vol. 1 ·No. 6 ·1981-11-00 ·Pages 527-42

Vater CA, Hahn JL, Harris ED

Abstract

Collagenase produced by monolayer cultures of rabbit synovial fibroblasts was purified from serum-free culture medium. Antiserum to the pure preparation was raised in sheep. The IgG fraction has been isolated and characterized and shown to be specific for collagenase. The antibody is capable of precipitating and inhibiting the activity of both latent and activated rabbit synovial fibroblast collagenase. Cross-reactivity with human antigen from rheumatoid synovial cell culture medium is described.

MeSH Terms

Animals Antibodies/isolation & purification Cells, Cultured Cross Reactions Fibroblasts/enzymology Immunodiffusion Immunoelectrophoresis, Two-Dimensional Microbial Collagenase/immunology Molecular Weight Rabbits Synovial Fluid/enzymology

Chemicals

Antibodies Microbial Collagenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Vater C A

Hahn J L

Harris E D

Article Info

Journal

Collagen and related research

Abbr.

Coll Relat Res

ISSN

0174-173X

DOI

10.1016/s0174-173x(81)80034-9

Published

1981-11-00

Pages

527-42

Language

English

Region

Germany

NLM ID

8102998

Subset

Grants

NIADDK NIH HHS · AM 14780 · United States

NIADDK NIH HHS · P60 AM20641-0251 · United States

External Links

PubMed source DOI: 10.1016/s0174-173x(81)80034-9

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Preparation of a monospecific antibody to purified rabbit synovial fibroblast collagenase.

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